Observation 224571: Bisporella citrina (Batsch) Korf & S.E. Carp.

Proposed Names

31% (2)
Based on microscopic features: none

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Eye3 = Observer’s choice
Eyes3 = Current consensus

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For my region
By: Stephen Russell (Mycota)
2017-12-06 22:49:22 PST (-0800)

probably 80-90% of the small yellow ascos fall into one of the three genera I mentioned. I collect and sequence all of them I encounter. I am sure more species/genera exist locally, but the three make up the significant majority of encounters.

GenBank has representatives from all of those genera
By: Stephen Russell (Mycota)
2017-12-06 22:39:56 PST (-0800)

It should not be too difficult to reliably get to genus with ITS alone.

I might have assumed the same
By: Danny Newman (myxomop)
2017-12-06 21:28:55 PST (-0800)

until I started uploading things to ascofrance and conversing about “bisporelloids” in general, and Hans Otto Baral resisted making any IDs, even to genus, without water microscopy. Calycella, Calycellina, Phaeohelotium and others are all varying degrees of macroscopically (and to some extent microscopically) similar, and many NA collections in herbaria labeled B. citrina have been misidentified.

I would also note
By: Stephen Russell (Mycota)
2017-12-06 21:14:29 PST (-0800)

I think I can identify all three reliably in the field with morphology, color, and texture.

The other common small yellow ascos I get
By: Stephen Russell (Mycota)
2017-12-06 20:55:45 PST (-0800)

are Ditiola and Orbilia. Both are reasonably defined in GenBank, so they can be ruled out for this observation.

GenBank has 11 records for Bisporella citrina, which all fall at the top of my BLAST results with 98-100% similarity. I think we can be reasonably certain about the identification.

All of this is true
By: Stephen Russell (Mycota)
2017-12-06 20:49:57 PST (-0800)

But with limited time for ascos, I have no issue trusting the reference until another more trusted source comes along. Look at it as an iterative process. Sets of matching observations are easy to locate and update.

I wonder
By: Danny Newman (myxomop)
2017-12-06 19:48:10 PST (-0800)

how many GenBank entries labeled as B. citrina are something else. No way of checking without borrowing whatever specimen a sequence may or may not correspond to, checking a paper in which that corresponding specimen was given detailed illustration, or referencing a corresponding MO post with micro of water mounts. The latter two are probably nonexistent for this species, and dried material prevents the checking of certain key characters only present in living, fresh material. It would be great to generate MO records which have both DNA and high quality micrographs. Those could then be the standard by which all other B. citrina GenBank entries are judged.

ITS sequence will be added soon.
By: Stephen Russell (Mycota)
2017-12-06 15:09:10 PST (-0800)
scoped? sequenced? closer photography? notes?
By: Danny Newman (myxomop)
2017-12-06 13:33:06 PST (-0800)

Created: 2015-12-06 16:07:01 PST (-0800)
Last modified: 2017-12-06 21:24:53 PST (-0800)
Viewed: 83 times, last viewed: 2018-04-28 22:17:45 PDT (-0700)
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