Observation 276929: Sphaeropsidales
When: 2017-05-19
No herbarium specimen
0 Sequences

Notes:
Cultured from http://mushroomobserver.org/274560.

Makes interesting black dots with a pore at the top.

Images

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One of the black dots magnified 400 times in Melzer’s

Proposed Names

29% (1)
Eye3 Eyes3
Recognized by sight

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Hard to say
By: Alan Rockefeller (Alan Rockefeller)
2017-06-20 16:21:05 CDT (-0400)

It’s usually kind of hard to tell with these types of fungi what is described. There will be some species in the literature, but it is hard to say how much of what is out there is described, or what the differences are between species.

Does that mean that this may be an undescribed species?
By: James Baker (cepecity)
2017-06-20 16:09:44 CDT (-0400)
MEA
By: Alan Rockefeller (Alan Rockefeller)
2017-06-20 14:51:48 CDT (-0400)

This was grown on standard MEA + green food coloring.

I tried to sequence this but the PCR didn’t work. I could try again with different primers or a fresh DNA extract – however often for obscure fungi like this the sequence only gets to genus or family as many of the BLAST matches are unnamed.

What media did you use to grow this fungus?
By: James Baker (cepecity)
2017-06-20 14:31:58 CDT (-0400)

Did you add morel extract to the media? The species shown in the link photo is a parasite on rust fungi. I wonder if a rust fungus extract was needed to produce the growth shown. Do you plan to sequence this fungus to check if it’s an undescribed species?

Thanks!
By: Alan Rockefeller (Alan Rockefeller)
2017-06-20 06:27:30 CDT (-0400)

The first picnidia pic in the link you posted is exactly what I am seeing here. It was cultured from Morchella snyderi.

Black dots may be pycnidia
By: James Baker (cepecity)
2017-06-20 02:41:32 CDT (-0400)

Black dots with an apical pore is how pycnidia are described, the spore forming sructure of COELOMYCETES. “pycnidia release their spores in a wet mass that is extruded out through the apical pore (ostiole)” from -http://website.nbm-mnb.ca/...

Similar culture plate appearance to Sclerotinia sclerotiorum cultures
By: James Baker (cepecity)
2017-06-20 01:48:28 CDT (-0400)

Observation 274560 is Morchella snyderi. Was this fungus found growing on the morels? The appearance of your photos of culture plates reminds me of photos of cultures of Sclerotinia sclerotiorum that I’ve seen such as this- https://www.researchgate.net/...
but Sclerotinia sclerotiorum is a plant pathogen.

Created: 2017-05-19 17:28:38 CDT (-0400)
Last modified: 2017-06-20 06:25:13 CDT (-0400)
Viewed: 68 times, last viewed: 2017-07-01 00:49:47 CDT (-0400)
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