NEW FEATURES! Read about them here.
Introduction
How To Use
How To Help
Donate
Feature Tracker
Send a Comment

Index A→Z
List Locations
List Projects

Latest:
 Changes by Users
 Images
 Comments
 Features and Fixes

Observations:
 Create Observation
 Sort by Date

Species Lists:
 Create List
 Sort by Date
 Sort by Title

Account:
 Login
 Create Account

Languages:
 Deutsch
 Ελληνικά
 English
 Español
 Français
 Polski
 Português
 Русский

Contributors
Site Stats
Translator’s Note

Colors from Black on White

Powered by:
Ruby on Rails
Preferred browser:
FireFox

Valid XHTML 1.0 Transitional

Observation: Hohenbuehelia atrocaerulea var. grisea (34367)
About Hohenbuehelia atrocaerulea var. grisea
When: 2010-02-12
Collection location: Los Trancos Preserve, Palo Alto, California, USA [Click for map]
Who: Douglas Smith (douglas)
Herbarium specimen available

Notes: Found on the bark of live oak.

The micro-shot is of the gill edge at 400x in KOH. The metuloid cystidia here are numerous. The cystidia are kinda interestingly encrusted, many encrusted in the top half, ending in a hard line.

Adding more micro details:

The second micro-shot is of the squashed gill edge at 1000x in congo red. Here are the smaller cystidia with a single projection.

The third micro-shot is of the squashed gill face at 1000x in congo red. Here are the encrusted metuloid cystidia.

The forth micro-shot is of spores from the gill at 1000x in congo red. The ave. spore size is : length – 7.16 +/- 0.43 (err 0.17) um, width – 5.77 +/- 0.58 um (err: 0.22) – q : 1.25 +/- 0.11, on 8 spores.

With the single projections to the smaller cystidia, from Funga Nordica this matches – H. grisea. It seems some sources have Hohenbuehelia atrocaerulea var. grisea and other have Hohenbuehelia grisea. And Index Fungorum call these synonyms. So, anyway this seems to be a good id, even though others seem to find this id fairly questionable or uncertain.

Proposed Names: Propose Another Name
Proposed Name User Community Vote
  douglas   86% (1)   Eye3Eyes3
Recognized by sight

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice Eyes3 = Current consensus
Comments: Add Comment

Created: 2010-03-17 14:50:58 WET (+0000)
By: Debbie Viess (amanitarita)
Summary: nice Doug.

OK, I am feeling the pressure to return to my scope…;)

Ironically, IMO, those encrusted cystidia actually showed up better in the unstained version!

202406

Created: 2010-03-10 19:29:53 WET (+0000)
By: else
Summary: cheilocystidia

Hohenbuehelias have two types of cheilocystidia – those big thick-walled ones with the crystals which take front page, but also, small one with 2 or 3 finger like appendages. you have to squash the slide a bit to get to see those properly


Created: 2010-03-10 16:44:45 WET (+0000)
By: Debbie Viess (amanitarita)
Summary: no worries Doug, we can share the pain…

I’ll use congo red on my examples of this one from San Diego.

202406

Created: 2010-03-10 16:15:36 WET (+0000)
By: Douglas Smith (douglas)
Summary: Didn’t use Congo Red

Well, there are a number of reasons. First perhaps, is that I wanted to see that the spores were hyaline, so I could tell that these weren’t Crepidotus with some tan or pigmented spores, and congo red could obscure that. Also I had been looking at Psathyrella and Inocybe lately (boy the life I lead!), and I wanted to be able to see if the cystidia walls were pigmented. And in the end, because I just hate to use congo red, I just do…

Now that I know that there are no pigmentation to look for at all, which I didn’t before hand, using congo red seems obvious. And I should try to use it in the future, if I get back to looking at these… which will probably never happen. And I’ll still hate to use congo red…

7181

Created: 2010-03-10 15:43:18 WET (+0000)
By: Dimitar Bojantchev (dimitar)
Summary: Cool stuff

Very cool stuff. Why not use a drop of a colorizer on the micro shot? Congo Red works great in such cases..

D.
71884


Created: 2010-02-28 14:12:02 WET (+0000)
Last modified: 2010-02-28 14:12:02 WET (+0000)
Viewed: 236 times, last viewed: 2012-05-09 02:57:46 WET (+0000)
Show Log