Observation 107668: Amanita sect. Validae (Fr.) Singer
When: 2012-08-29
Herbarium specimen reported

Proposed Names

-13% (4)
Eye3
Recognized by sight
46% (2)
Recognized by sight: not brunnescens, at any rate.
ret
27% (1)
Based on microscopic features: See comments, below.
ret
54% (1)
Eyes3
Based on chemical features

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
We obtained DNA (ITS and LSU sequences) from this material, Steve.
By: R. E. Tulloss (ret)
2014-03-04 13:43:48 CST (-0500)

These sequences were somewhat similar to those from MO 109802 and 110440, but suggest (at least for the moment) that this is a separate species.

Rod

From my experience, David,…
By: R. E. Tulloss (ret)
2013-05-28 19:42:25 CDT (-0400)

I’d say you are correct about two stage drying. When I get material home from the field, I get it written up (if necessary) as fast as possible and into the dryer. I actually do a sort of triage. Processing small, fragile, and rare things first.

R

I’ve been reading…
By: Dave W (Dave W)
2013-05-28 18:54:47 CDT (-0400)

some of your comments, Rod, about the best way to preserve material for study. I gather that it’s best to get the stuff into the dehydrator asap, and then store in a way that will minimize rehydration due to high ambient humidity.

Often I have pre-dried material by hanging it in a sunny south-facing attic window, then finishing in the dehydrator. This method seems to result in nice preservation of macro-traits. But it seems this is not best for preserving spore features and/or molecular features.

I’m sending you a sporograph.
By: R. E. Tulloss (ret)
2013-05-28 15:46:22 CDT (-0400)

Steve,

I think that the last photo you took indicates that the specimen aged a little before it got into the dryer. Slow drying or situations resulting in delay before drying both yield some drying/aging at ambient temperatures and humidity. This will drop spore length and the Q value of the spores at least to some degree…maybe more. Hence the fact that the sporograph of the MO posted material is smaller and “low and inside” (suddenly was struck by the baseball analogy) would be indicative of drops in spore size and spore Q. That seems to fit the situation in this case.

Am I correct in thinking there was a delay before drying?

Very best,

Rod

The spores are amyloid and broadly ellipsoid to ellipsoid. EDIT
By: R. E. Tulloss (ret)
2013-05-28 14:47:18 CDT (-0400)

Given the photographs, I think it belongs in sect. Validae all right; however, the spores (and the somewhat rooting bulb…tip cut off unfortunately) make it clear that this is not brunnescens. Given the fact that Steve collected at least two specimens of A. submaculata last year (thank you, again), it might be that species. I’ll do some more detailed measurements on the spores and see where that goes. I noticed some reddish staining on the warts on the cap.

Amanita submaculata often has no warts left on the cap; however, that species does show reddish staining around the edges of insect “bite” holes in the cap skin and on the stem and bulb. In addition submaculata has a graying volva that sometimes appears weakly felted and a virgate pileus. Both present here. There are areas of the cap that seem to have a greenish tint, but that may have been induced by the lighting in a leafy forested area.

Back to you in awhile.

Rod

Steve, thanks for sending this one.
By: R. E. Tulloss (ret)
2013-05-28 12:49:16 CDT (-0400)

I’ll give a quick try at figuring out what it might be.

More later,

R

Created: 2012-08-29 19:11:35 CDT (-0400)
Last modified: 2014-03-04 13:41:17 CST (-0500)
Viewed: 73 times, last viewed: 2016-06-06 00:39:21 CDT (-0400)
Show Log