Observation 108540: Limacella “sp-L-CMP0152” Tulloss cryptonom. temp.
When: 2012-08-22
No herbarium specimen

Notes: In grass with aspen in mixed conifer.

Proposed Names

-19% (4)
Eye3
Used references: States. Mushooms and Truffles of the SOuthwest. 1st ed
Based on microscopic features
Based on chemical features
44% (3)
Recognized by sight
ret
54% (1)
Recognized by sight: Slimy stipe.
ret
81% (1)
Eyes3
Based on microscopic features: See comment below.
Based on chemical features: nrLSU sequence. See comment below.

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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A nuclear ribosomal large subunit sequence of good quality was obtained…
By: R. E. Tulloss (ret)
2016-11-14 08:41:44 PST (-0800)

from this material.

It has just been OK’d for releases by GenBank. Its GB accession number is KX868001. It should be available on line in a few days.

Very best,

Rod

Based on spores, clamps, and overall appearance of exsiccata….
By: Cristina Rodríguez-Caycedo (Crispeta)
2013-06-28 16:00:03 PDT (-0700)

…it seems possible we are looking at Limacella sp-CMP0152. We first compared spore measurements from the largest pileus in your collection to all the spore data of section Lubricae (http://www.amanitaceae.org/...). After measuring the default minimum of 20 spores, the following data was obtained: [20/1/1] (4.0-) 4.2-5.5 × (3.0-) 3.1-4.1 (-4.2) μm; Q = (1.20-)1.27-1.44(1.61).

With the use of the WAO sporograph tool we found the closest match to be L. sp-CMP0152, which consequently, is also found in Arizona.
The spores of your material are inamyloid, broadly ellipsoid to ellipsoid (rarely elongate), and some were decorated with fine warts. This echinulate appearance was only evident with the use of Nomarski. You will find that L. sp-CMP0152 from Cochise Co. (AZ) was dominantly echinulate and noticeable even without the use of Nomarski.

The macromorphological description of L. sp-CMP0152 was only based on exsiccata, because a good description of the fresh material was not available. So we compared your dried material with the information on WAO (http://www.amanitaceae.org/?Limacella%20sp-CMP0152) and we found them to match well. Some of the characters they share are color of pileus, color of stipe, gill attachment, and shape of lamellulae. Also, there were prominent (kidney-like) clamps at the bases of basidia. In order to observe them, a squash in KOH+Congo Red was necessary.

Hope this was helpful and informative,
Cristina

(From the Roosevelt, NJ Team)

Hello, Erik.
By: R. E. Tulloss (ret)
2013-06-28 07:45:27 PDT (-0700)

We have submitted a sample of our material for DNA sequencing, but do not have a schedule for receiving the sequences. A quick examination of the material along with your photographs and field notes gets us this far. This material (because of the slimy stem) belongs in Limacella sect. Lubricae. It would very likely be called L. illinita if you found a picture of it in a North American book; however, that would not be correct. This is something distinct to North America.

Based on collections from Cochise Co. (Chiricahua Mtns.) in Arizona, we have good evidence that there are multiple, pallid, illinita-like taxa in the U.S. Southwest. Their ranges may extend northwest into California and south into Mexico. The material of pallid limacellas that we have from Florida and the Gulf Coast appears to belong in yet another taxon. This latter may already have a name…L. subillinita. We have sent samples of all the limacella material in our herbarium, some type material, and material on loan from Michael Kuo to a laboratory that has agreed to work with us on sequencing as many limacellas as possible world wide. As I said before, we have sent the samples; but do not have a schedule for receiving sequences (if they can be obtained from the samples).

We have not had time to work up all our material microscopically, and the microscopy on your material has not started.

This is the current status, Erik.

Very best,

Rod

The package of dried material has arrived.
By: R. E. Tulloss (ret)
2012-09-20 13:24:20 PDT (-0700)

Thank you very much, Erik.

I will post here what we learn from your collection.

Very best,

Rod

I don’t think that this is the European L. illinita,…
By: R. E. Tulloss (ret)
2012-09-03 18:47:01 PDT (-0700)

which has been posted on this site. I am very interested to know if you have dried or are willing to dry the specimens in your photos. If the answer is “yes” in either case, would you be willing to send the dried material to me?

My postal address is given here:

http://www.amanitaceae.org?About+Our+Editors

Thank you,

Rod Tulloss

Created: 2012-09-03 18:30:17 PDT (-0700)
Last modified: 2016-11-14 08:58:18 PST (-0800)
Viewed: 131 times, last viewed: 2016-11-14 12:49:29 PST (-0800)
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