Observation 113388: Limacella guttata (Pers.) Konrad & Maubl.

When: 2012-10-14

Collection location: Zürich-City, Switzerland [Click for map]

47.0° 8.0° 449m

Who: Sporulator

Specimen available

growing in a park under Fagus sylvatica


Limacella guttata mycelium on agar
Limacella guttata mycelium on agar
Limacella guttata mycelium on agar

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Good morning, Gerhard.
By: R. E. Tulloss (ret)
2014-11-04 04:59:39 PST (-0800)

In the last week we obtained a small flood of DNA data concerning Limacella from Jozsef Geml and Tatiana Semenova in Leiden. The material of this observation contributed our obtaining a good set of data for L. guttata. I am very grateful for your contribution of the collection associated with this observation.

Very best,


Very nice.
By: R. E. Tulloss (ret)
2014-07-29 07:16:27 PDT (-0700)

The video gives a real sense of the size of the fruiting.

I have never seen a fruiting of Limacella of this size.

Very best,


By: Sporulator
2014-07-29 06:45:01 PDT (-0700)
By: Sporulator
2012-10-17 08:27:05 PDT (-0700)

It is the city of Zürich. Click on “collection location” and you will see the exact place (satellite map) where they grow at the moment. Geo-data: 47º 25’ 15,63’’ N – 8º 32’ 46,49’’ E

Thanks for permission…
By: R. E. Tulloss (ret)
2012-10-17 07:44:44 PDT (-0700)

even though it is not required.

In your geo-data does Zürich refer to the Canton or both the Canton and the City? (I need that for my herbarium labels.)

One of your images will shortly appear on


Please excuse our low level of detail for the species we are still gathering data.

Very best,


By: Sporulator
2012-10-17 07:34:27 PDT (-0700)

Of course everyone may use my photos.

Thanks for the Mycologia link!

In my experience, the pH value is a very important parameter.
Calcium is essential for many mushroom species, like Guepinia helvelloides or Lyophyllum
decastes, for example. But because most fungi prefer to grow under acidic conditions, the Calcium must
be bound to a (preferably organic) acid, like tartaric acid or citric acid, in order to keep the pH value of the agar medium low. I have learned that there is a significant difference if I grow a species at pH 4.0 or pH 5.5
on agar.

I will keep you updated about my cultivation trials via Email.



I would like to use one of your images on WAO site.
By: R. E. Tulloss (ret)
2012-10-16 08:57:10 PDT (-0700)

I know that it is not strictly necessary to ask your permission, but I am old-fashioned. May I use one of the images of unopened specimens?

Very best,


Also, ….
By: R. E. Tulloss (ret)
2012-10-16 07:22:00 PDT (-0700)


The first issue of Mycologia for 2012 has as “the cover girl” Amanita thiersii. The article on that species in that issue will give you more information about the growing of the basal amanitas in culture.

Very best,


It would be very interesting to know if varying the pH in the culture would affect …
By: R. E. Tulloss (ret)
2012-10-16 07:18:04 PDT (-0700)

growth rate or other characteristics (e.g., the ability to fruit). Wolfe achieved fruiting of some free-living amanitas in culture. No one has ever fully succeeded with fruiting of an ectomycorrhizal amanita in culture … to my knowledge…despite the fact that some taxa grow qrow quite luxuriant mats of hyphae in culture as has been known for several decades.

I notice that soil pH is emphasized much more in European literature on macrofungi than it is in North American literature. I wonder if pH is an equally important element in the growth of all macrofungi. Could it be terribly important for a species of Hygrophorus (just as an example), but not critical for a species of Limacella or even a second Hygrophorus?

Again, I wish you good luck. I will be very interested in your results and would be pleased if you would stay in contact with me regarding your work. If you contact me through MO or http://www.amanitaceae.com?Contact+us , I will see your email address and can interact with you outside of the MO comment boxes.

Very best,


By: Sporulator
2012-10-16 05:39:59 PDT (-0700)

Very interesting informations, thanks for this! That’s all new to me.
Interestingly, in Switzerland Limacella guttata grows only in calcareous soil, sometimes together with Tuber aestivum. I have to consider that when preparing a culture medium.

I will send you dried specimens for the herbarium.


The cultvation trial is a great idea.
By: R. E. Tulloss (ret)
2012-10-15 12:54:10 PDT (-0700)

Regarding culture of members of the Amanitaceae I suggest that you take a look at the recent paper by B. Wolfe et al. (appeared in early summer of this year in PLoS ONE) concerning evolution of the ectomycorrhizal life style in Amanita.

In that article Wolfe reports experiments demonstrating that the free-living amanitas (basal to the genus Amanita and probably sharing a common ancestor with all or part of Limacella) could, among other things, live on pure cellulose or sterilized grass clippings from lawns. It would be interesting to see if the genes for breakdown of cellulose in the free-living amanitas are very similar to those in the limacellas. It would be interesting to look at evolution of cellulase genes within the Amanitaceae up to the point of disappearance of several of said cellulases in almost all examined extant ectomycorrhizal Amanita lineages…that is if the possibly quite large impact of extinction on Limacella permits any useful/meaningful data to be assembled.

Good luck.

And, yes, I’d be delighted to have a few dried specimens from your collection.

My mailing address is here:


Very best,


By: Sporulator
2012-10-14 22:42:48 PDT (-0700)

Some specimens are in my fridge at the moment.
I will start a cultivation trial on agar. No problem to dry some specimens.


I believe that all Limacella species are thought to be saprotrophic.
By: R. E. Tulloss (ret)
2012-10-14 13:29:45 PDT (-0700)

Did you happen to dry and retain these specimens?

Rod Tulloss

By: Sporulator
2012-10-14 08:48:38 PDT (-0700)

Wikipedia says, that this is a saprophytic species. True?

Video: http://www.youtube.com/watch?v=fQsWBExv_co

Created: 2012-10-14 08:42:53 PDT (-0700)
Last modified: 2018-01-02 08:23:55 PST (-0800)
Viewed: 463 times, last viewed: 2018-03-18 00:51:20 PDT (-0700)
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