Observation 114279: Panaeolina castaneifolia (Murrill) Bon
When: 2012-10-22
Collection location: Washington, USA [Click for map]
No herbarium specimen

Notes: Resembles a dark, larger version of Panaeolina but more closely resembles a Panaeolus species, perhaps Panaeolus cinctulus or another Panaeolus.

Images

274797
cover.JPG
274798
P1110160.JPG
274799
P1110162.JPG
274800
P1110164.JPG
274801
P1110166.JPG
274802
P1110171.JPG
274803
P1110173.JPG
274804
P1110174.JPG
274805
P1110176.JPG
274806
P1110178.JPG
274850
Roughly ornamented spores.tiff
274851
Basidia.tiff
274852
A cheilocystidium.tiff
274853
Cheilocystidia.tiff
274856
Pileus pileipellis.tiff
274857
Pileus trama.tiff
274888
Gill trama 1.tiff
274889
Gill trama closer up.tiff
274890
4-spored Basidia.tiff
274891
A spore is questioned under the scope.tiff
274894
Spores in distilled water (from print).tiff
274895
Pileus epicutis cells vary from globose to subglobose to bean-shaped .tiff
274896
Pileus epicutis cells vary dramatically in size.tiff
274897
Pileus epicutis from a distance.tiff
274908
Oil droplet.tiff
274909
Oil drops.tiff

Proposed Names

-48% (3)
Recognized by sight
44% (2)
Eye3
Recognized by sight
-31% (3)
Recognized by sight
62% (3)
Eyes3
Recognized by sight: In grass, cap wrinkled, dark brown when moist. Gills crowded.
Used references: http://myco-tek.org/...
Based on microscopic features: Spores without oil droplets and conspicuously roughened, basidia 4 spored, cheilocystidia fusoid-ventricose

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Common
By: Byrain
2012-10-24 04:45:49 PDT (-0700)

I suspect this type is common, but overlooked. Its not hard to find in my area at least…

Excellent Micrographs …
By: Stephen (Ιερονυμοσ)
2012-10-23 21:56:54 PDT (-0700)

Your photographic and microscopic skills are most impressive. You have inspired me to clean off my lenses! Excellent find as well, rather rare.

Light!
By: lightworkerpeace (gsharpnolack)
2012-10-22 21:47:08 PDT (-0700)

Thank you, sir!

Gsharp
By: Rocky Houghtby
2012-10-22 21:45:26 PDT (-0700)

Those micrographs are outstanding

Regarding identification
By: lightworkerpeace (gsharpnolack)
2012-10-22 21:39:07 PDT (-0700)

As far as species goes, I will leave that to peer consensus. I have doubts that this is Panaeolina castaneifolia but Alan is typically solid with identification. I will attempt to photograph this species again tomorrow and hopefully throughout the week. After all, they are growing very, very closely to P. stuntzii.

If you can think of any other distinguishing factors feel free to suggest them.

That worked.
By: lightworkerpeace (gsharpnolack)
2012-10-22 21:33:47 PDT (-0700)

It’s a small comparison to the ones that appear not to have an oil drop, but I’ve added two images showing three spores that did have them.

oil drops
By: Byrain
2012-10-22 21:32:42 PDT (-0700)

In the P. foenisecii type, most spores will have 1-2 oil drops like your picture of a single spore, in the P, castaneifolia type, most spores will not. Outside of that, P. castaneifolia will only have 4-spored basidia & epicutis cells reported as 25-35 μm and P. foenisecii can have 2 or 4 spored basidia & epicutis cells reported as 15-25 μm.

If the spores had oil drops
By: Alan Rockefeller (Alan Rockefeller)
2012-10-22 21:19:19 PDT (-0700)

They would be visible in KOH or water. I think the spore pic is good enough to say that they don’t have oil drops. Check again with just your eyes with nice bright lighting at 1000x, you should be able to see them pretty well if they were there.

Scope results
By: lightworkerpeace (gsharpnolack)
2012-10-22 21:16:34 PDT (-0700)

Hi. The measurement of the width of the epicutis cells might be counter productive. They vary in size super-dramatically. I was willing to measure a few of them until I saw this difference. In my mind measurements of this item would not lend to identification, but rather confusion.

As for the basidia, they all appear to be four-spored. There was one instance where I thought I saw a five-spored basidium, but I couldn’t be 100% certain. The spores may or may not have oil drops. A different stain would be necessary (not KOH or congo red). I’m not sure I have the right stain for the job.

Epicutis cells
By: Byrain
2012-10-22 19:55:57 PDT (-0700)

Can you measure the width for the cells in he epicutis? They should be globose.
And confirm that the spores are without oildrops & that there are no two-spored basidia?

Created: 2012-10-22 18:00:20 PDT (-0700)
Last modified: 2012-10-23 21:58:17 PDT (-0700)
Viewed: 180 times, last viewed: 2016-07-14 15:53:27 PDT (-0700)
Show Log