Observation 120787: Inocybe sambucina (Fr.) Quél.
When: 2012-10-05

Notes: Pale cream Inocybe sp. growing from the ground in mixed woods alongside the Bingham Rest Area off Route 201 in Bingham, ME. Smell was mushroomy/spermatic. Spore print was brown. Spores are smooth and elongated to cylindric (see photomicrograph). The 20 spore average was 8.8 × 4.0 microns, Qm = 2.18. Encrusted metuloid cystidia are present on gill surface.

Species Lists

Images

296149
296150
20 Spore average was 8.8 × 4.0 microns, Qm = 2.18.
296151
DIC photomicrograph of encrusted cystidia in 10% KOH in glycerol/water (1:10).
296152

Proposed Names

55% (1)
Recognized by sight
Based on microscopic features: Encrusted metuloid cystidia are present on gill surface.
82% (1)
Eye3 Eyes3
Based on chemical features: ITS sequence has 99% similarity with Inocybe sambucina

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Thanks Irene!
By: Linas Kudzma (baravykas)
2013-04-11 09:23:27 CDT (-0400)

My Observation 109443 is larger and looks somewhat similar, but is microscopically very different. Still waiting for some DNA results for Obs 109443.

Interesting
By: Irene Andersson (irenea)
2013-04-11 02:45:26 CDT (-0400)

Spores and cystidia are a good match with sambucina. The colours too – greenish yellow hue on the cap is a typical character. It’s no doubt that this is a close one, but not as large and sturdy as I’m used to see it.
Still, maybe close enough to be the same..?

Closest match of my ITS sequence …
By: Linas Kudzma (baravykas)
2013-04-10 17:40:03 CDT (-0400)

for this Observation is GeneBank Accession number AM882757.2 Inocybe sambucina 18S (partial), ITS1, 5.8S rRNA gene, ITS2 and 25S rRNA (partial), specimen voucher SJ01002

I’ll post details this evening.
By: Linas Kudzma (baravykas)
2013-04-10 15:46:01 CDT (-0400)
ITS sequence has 99% similarity with Inocybe sambucina
By: Oluna & Adolf Ceska (aceska@telus.net)
2013-04-10 15:29:12 CDT (-0400)

Can you be more specific and post what collection of Inocybe sambucina you are referring to?

Created: 2012-12-22 15:43:34 CST (-0500)
Last modified: 2013-02-11 13:08:13 CST (-0500)
Viewed: 222 times, last viewed: 2016-12-03 04:56:33 CST (-0500)
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