Observation 127432: Inocybe fastigiella group
When: 2012-09-09

Notes: Robust brown colored Inocybe sp. growing from the ground in mixed woods. Caps are 5-3 cm in diameter and are darkly fibrous. Stipe ranges in color from cream to brown near the base. Spore print was brown and spores are smooth and bean-shaped. The 20 spore average size was 8.3 × 4.5 microns and average Q=1.86. Cystidia are infrequent and possibly only closely grouped cheliocystidia. The cystidia are thin walled, not encrusted and remain colorless in 5% KOH. See photomicrographs for shapes of spores and cystidia.

Proposed Names

55% (1)
Recognized by sight
Based on microscopic features
60% (2)
Recognized by sight
82% (1)
Eye3 Eyes3
Recognized by sight
Based on microscopic features
Based on chemical features

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Eye3 = Observer’s choice
Eyes3 = Current consensus


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ITS sequence is 99% similar to ..
By: Linas Kudzma (baravykas)
2014-12-26 23:12:05 CET (+0100)

GenBank JQ801399 “Inocybe aff. fastigiella 2”. See Observation 75596 for a collection whose sequence matches “Inocybe aff. fastigiella 1”. Both are very similar in appearance and these temporary names are Dr. P.B. Matheny’s.

Inocybe aff. fastigiella
By: Linas Kudzma (baravykas)
2013-02-11 20:41:05 CET (+0100)

As indicated by a 98% similarity with “Inocybe aff. fastigiella” in GenBank.

Danny, I can definitely give you advice ..
By: Linas Kudzma (baravykas)
2013-02-02 23:02:23 CET (+0100)

… and don’t hesitate to ask. We can continue this conversation privately.

However, I will say here that some remarkably good optics and older scopes are out there. The main reason is that all modern scopes, from the Chinese knockoffs to top of the line offerings from Zeiss, Olympus and Nikon now use infinity corrected objectives. My entire setup uses 160mm tube length objectives, which allowed me to assemble a system that many years ago was untouchable by anyone without serious money. Some such older 160 TL objectives and related older optics (condensers, photo projection eyepieces, etc) rival anything available now, but are a fraction of the cost used because they only work with the “obsolete” 160mm tube length scopes. Of course, I had to spend years collecting and you have to navigate many unscrupulous folks and items in poor condition. It’s never one-stop shopping.

My advice for someone starting to assemble such a system is to pick one (like Zeiss), buy a basic stand and start adding to it. Join some on-line microscopy groups. There are many very knowledgeable and helpful folks out there.

By: Danny Newman (myxomop)
2013-02-02 21:13:26 CET (+0100)

Don’t spare me, I beg of you. I think I can speak for more than myself when I say that this kind of information would be an asset to more users than just me, regardless of the information’s visibility tucked away inside a single observation.

I’m attempting, somewhat unsuccessfully, to navigate the used microscope market… alone. One Rocky Mountain Microscope Corp., a company I enlisted in building me a microscope back in October, has been yanking my chain for over four months with requests for permanent slides and recipes for KOH, only to finally send out “test images” that look like they were taken on a Sony Handycam in the middle of an electrical storm.

I dream of Nomarski/DIC, but I’m working with a brightfield budget. My realistic ideal is a trinocular LED w/ Koehler and the flattest, sharpest objectives that fit inside a $2000 spending limit, with the versatility to easily hot swap components if/when I can afford more expensive doodads. Are there people who specialize in leading the deaf, dumb and blind likes of me through this purchasing process?

Thanks Danny …
By: Linas Kudzma (baravykas)
2013-02-02 14:46:53 CET (+0100)

My microscopy setup is a time warp and the result of 40 years of obsessive collecting and upgrading. My main scope is a Zeiss Universal using a hybrid arrangement of Zeiss and Leitz optics. All were absolutely the best money could buy … in the 1970’s. Now mortals can afford them on the used market, if they can be found. The DIC accessories (Nomarski prisms, Pol analyzers) were the very first commercialized by Zeiss. Photos are with a Canon EOS 40D attached via a homemade rig using tubes/spacers from Edmund Scientific. I could write a book about all this, but I’ll spare you.

By: Danny Newman (myxomop)
2013-02-02 09:20:01 CET (+0100)

what is your scope setup? these images are pretty impressive.

Thanks Richard …
By: Linas Kudzma (baravykas)
2013-02-02 03:56:58 CET (+0100)

microscopy/photomicrography has been a passion for even longer than mycology and they are now inseparable. Yes, some variety of I. fastigiata is almost certain.

I have 15 of my Inocybes (including this) being sequenced as we speak. Soon I’ll have more data.

beautiful micro…
By: Richard and Danielle Kneal (bloodworm)
2013-02-02 03:42:46 CET (+0100)

Inocybe fastigiata var. microsperma is a possibility here i think.

Created: 2013-02-02 03:22:14 CET (+0100)
Last modified: 2014-12-26 23:12:29 CET (+0100)
Viewed: 163 times, last viewed: 2016-10-25 12:16:21 CEST (+0200)
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