Observation 128946: Psilocybe subaeruginascens Höhn.
When: 2013-01-12

Notes: Found by Shrine, macroscopic pics by Shrine, micrographs by Alan Rockefeller. From this thread.

Growing in a tight cluster in between some flowers in a soil/manure mix.

Pileus subumbonate, hygrophanous, with a striate margin when moist. Stipe with an annulus.

For the stained micrographs, crystal violet was used. Unstained micrographs are mounted in KOH.

Pileipellis with a gelatinized hyaline layer with elements 2 – 4 µm wide. Pileus trama with irregularly shaped cells, light brown, approx 9 – 20 µm. Hymenophoral trama with irregularly shaped elongated elements, hyaline in KOH, 12 – 18 × 15 – 30 µm. Subhymenium like hymenophoral trama but with smaller elements, 4 – 6 × 5 – 10 µm.

Basidia 20 – 32 × 7 – 8 µm (10 measured), most with granular contents but some are hyaline, 2 and 4 spored basidia present. Sterigmata 3 µm.

Cheilocystidia very common, hyaline, occasionally with a refractive apex, and of two types: (20 measured)
Type A: fusoid-ventricose, rarely subcapitate, 15 – 22 × 6 – 8 µm
Type B: mucronate with an obtuse apex, occasionally with a chunk missing from the side giving it the appearance of a folded over paper bag, 18 – 26 × 8 – 11 µm.

Pleurocystidia scattered, hyaline and of two types (20 measured) –
Type A: fusoid-ventricose, 20 – 22 × 6 – 8 µm
Type B: mucronate with an obtuse apex, occasionally with a chunk missing from the side giving it the appearance of a folded over paper bag, 19 – 22 × 8 – 11 µm

Spore statistics: (30 measured)

8.5 [9.3 ; 9.6] 10.4 × 6 [6.6 ; 6.8] 7.3 × 5.2 [5.4 ; 5.6] 5.8 µm
Q = 1.2 [1.4 ; 1.5] 1.6 ; N = 30 ; C = 95%
Me = 9.4 × 6.7 µm ; Qe = 1.4

Species Lists

Images

311047
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Spores 1000x
311034
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Pileipellis scalp section 400x
311035
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Pileipellis scalp section 400x
311036
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Pileipellis scalp section 400x
311037
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Pileus cross section 40x
311038
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Pileipellis cross section 400x
311039
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Pileipellis cross section 400x
311040
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Cheilocystidia 400x
311041
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Lamellar trama 400x
311042
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Basidia, subhymenium and pileus trama 400x
311043
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Cheilocystidia 1000x
311044
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Cheilocystidia 1000x
311045
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Cheilocystidia 1000x crush mount
311046
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Spores 1000x
311048
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Spores 1000x with measurements
311049
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Pleurocystidia type A, basidioles and a basidium, 1000x
311050
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Copyright © 2013 Shrine
311051
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Copyright © 2013 Shrine
311052
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Copyright © 2013 Shrine
311053
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Copyright © 2013 Shrine
311054
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Gill cross section showing lamellar trama on left, subhymenium in the middle and basidioles on the right, 1000x
311060
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Pleurocystidia type B 1000x

Proposed Names

7% (2)
Eye3
Recognized by sight: Dried basidiomata are the same color as Psilocybe cubensis, as described in Guzman 1995. Stipe with annulus.
Used references: Supplement to the monograph of the genus Psilocybe. By Gaston Guzman, 1995.
Based on microscopic features: Spore shape, length, width and thickness matches. Pileipellis matches. Some of the cheilocystidia match. Pleurocystidia is not mentioned in Guzman 1995.
-16% (2)
Eye3
Recognized by sight: Looks like cubensis when dried.
Used references: The Genus Psilocybe by Gaston Guzman
Based on microscopic features: Spore shape is subrhomboid vs. subhexagonal. Spore size is close but slightly smaller than reported for P. subcubensis. Basidia matches. Both forms of cheilocystidia match including subcapitate heads. Both forms of pleurocystidia match.
Based on chemical features: Staining blue where damaged
-16% (2)
Eye3
Recognized by sight: A synonym of P. subcubensis
89% (2)
Eyes3
Recognized by sight
Based on microscopic features

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Not by a long shot
By: Peter G Werner (pgwerner)
2013-03-05 07:24:57 CET (+0100)

“Psilocybe ovoideocystidiata es un sinónimo con P. subaeruginascens”

It most certainly is not, nor is it a synonym of P. septentrionalis (even though it is a close relative), nor is P. septentrionalis a synonym of P. subaeruginascens.

I have the spores of both on the same slide in this image:

http://mushroomobserver.org/image/show_image/313808?obs=129758&q=16qxj

The difference even in the spores could not be clearer.

Psilocybe subaeruginascens
By: A. Cortés-Pérez (Alonso)
2013-03-05 06:49:49 CET (+0100)

Psilocybe ovoideocystidiata es un sinónimo con P. subaeruginascens

Looks like actual Psilocybe subaeruginascens
By: Peter G Werner (pgwerner)
2013-03-05 06:32:50 CET (+0100)

Looking at the very thick-walled spores, that really does look like Psilocybe subaeruginascens Höhn. rather than Psilocybe subaeruginascens sensu Guzmán, aka Psilocybe septentrionalis.

Carefully compare this to Horak and Desjardin’s description, because they made a careful study of both the holotype and a more recently collected topotype that was in much better shape.

Created: 2013-02-19 00:26:07 CET (+0100)
Last modified: 2013-03-05 06:33:13 CET (+0100)
Viewed: 363 times, last viewed: 2016-12-03 04:07:57 CET (+0100)
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