Observation 129454: Pluteus primus Bonnard
When: 2013-02-24
( 250m)

Notes: Found on the ground in Monterey Cypress debris.

Pleurocystidia 84 – 94 × 14 – 19 µm (3 measured). The larger pleurocystidia without apical projections in the second picture is 27 µm across.

Cheilocystidia and pleurocystidia abundant, with horns. Cheilocystidia measured 56 × 17.

Pileipellis elements 6 – 12 µm wide, with frequent clamp connections.

Spore measurements:

6.7 [7.4 ; 7.7] 8.4 × 5.1 [5.5 ; 5.8] 6.3 µm
Q = 1.2 [1.3 ; 1.4] 1.5 ; N = 28 ; C = 95%
Me = 7.6 × 5.7 µm ; Qe = 1.3

Images

312819
pluteuscheilo60x.jpg
Cheilocystidia 60x
312704
pluteuscervinus.jpg
Spores 1000x
312705
IMG_0504.JPG
312706
IMG_0502.JPG
312707
IMG_0501.JPG
312708
IMG_0499.JPG
312709
IMG_0498.JPG
312710
IMG_0497.JPG
312711
pluteuscervinus-measure.jpg
Spores 1000x with measurements
312712
IMG_0511.JPG
Lamellar cystidia 400x in squash mount stained with crystal violet
312721
pluteus-pleuro.jpg
Pleurocystidia 400x
312722
pluteuspleuro2.jpg
Pleurocystidia 400x
312731
pluteus-cheilo.jpg
Cheilocystidia 400x
312734
IMG_0551.JPG
Pileipellis scalp section 100x
312735
IMG_0553.JPG
Pileipellis scalp section 400x
312736
IMG_0560.JPG
Pileipellis 1000x with clamp connections
312737
IMG_0559.JPG
Pileipellis 1000x with clamp connections
312738
pluteus-clamps.jpg
Pileipellis 1000x with clamp connections
312739
IMG_0566.JPG
Pileipellis cross section 100x
312740
IMG_0571.JPG
Pileipellis cross section 100x
312741
IMG_0567.JPG
Pileus trama 400x
312742
IMG_0570.JPG
Lamellar trama 100x
312743
IMG_0573.JPG
Pileipellis cross section 400x
312820
pluteus3.jpg
Cheilocystidia 20x
312822
pluteuscheilo20x-dic.jpg
Cheilocystidia 20x
312823
pluteuscheilo20x-dic2.jpg
Cheilocystidia 20x
312824
pluteusspores60x.jpg
Spores 60x
312834
pluteus_cystidia_60x_edf.jpg
Cheilocystidia 60x
315095
IMG_1354.pixi.jpg
Cheilocystidia 400x
315096
IMG_1355.JPG
Cheilocystidia 400x

Proposed Names

43% (2)
Recognized by sight
Used references: FAN2
Based on microscopic features
51% (2)
Eye3 Eyes3
Recognized by sight

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Pluteus primus
By: Alfredo Justo (Fredo)
2014-04-01 10:54:31 CDT (-0400)

considering habitat, geographical origin, presence of clamps and spore size Pluteus primus is a likely candidate

as Else pointed out the photos labelled as cheilocystidia actually represent pleurocystidia; keep in mind that in this group the metuloid pleurocystidia occur up to the very beginning of the lamella edges, sometimes right next to the cheilocystidia. Pluteus primus has longer a narrower cheilocystidia compared to other species in this group, so if you are able to check those that would be helpful to confirm ID

morphological differences among the species in section Pluteus are usually very subtle, and it will not be always possible to give names to some collections without molecular data

DIC Micrographs
By: Alan Rockefeller (Alan Rockefeller)
2013-02-26 20:48:36 CST (-0500)

Are from Merritt College.

Alan
By: Danny Newman (myxomop)
2013-02-26 19:13:44 CST (-0500)

where are these DIC micrographs from?

I tripple checked the cheilocystidia
By: Alan Rockefeller (Alan Rockefeller)
2013-02-26 07:45:41 CST (-0500)

It does often have apical projections.

The pic labeled cheilocystidia is from a gill edge.

Pluteus cervinus does not have clamps
By: else
2013-02-25 13:55:39 CST (-0500)

but other species that look very similar do have clamps in the pileipellis.

This is a complex of species; all these species are brown (in various tinges) and have thick-walled horned pleurocystidia.

The cheilocystidia are in all these species thin-walled and clavate – the picture labled cheilocystidia shows pleurocystidia.

Why is this P. cervinus then?
By: Byrain
2013-02-25 11:19:12 CST (-0500)

I understand that species should lack clamps and doesn’t have thick walled cheilocystidia with apical horns. You can find the FAN2 key for Pluteus along with an illustration showing the non-horned thin walled cheilocsytidia P. cervinus should have at google books.

http://pdfcast.org/paid/9789061919711

It has a lot of clamp connections
By: Alan Rockefeller (Alan Rockefeller)
2013-02-25 07:00:55 CST (-0500)

in the pileipellis.

Clamps present?
By: Byrain
2013-02-24 23:44:41 CST (-0500)

I think I see one in the first pleurocystidia picture.

clamps?
By: else
2013-02-24 21:48:30 CST (-0500)

one of the important characters to distinguish species in this complex is the absence or presence of clamp connections in the pileipellis.
Pluteus cervinus in the strict sense does not have them.

and secondly, look again at the cheilocystidia. all these species have thin-walled clavate cheilocystidia without horns.

and the pileipellis of the fruitbody in the picture looks wrong for P. cervinus. it looks like a hispidoderm, not a cutis.
what do the cystidia look like? are the pleurocystidia thick-walled with horns, or thin-walled with little thingies at the top?

Created: 2013-02-24 21:32:08 CST (-0500)
Last modified: 2014-10-23 14:03:45 CDT (-0400)
Viewed: 287 times, last viewed: 2016-10-27 06:07:06 CDT (-0400)
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