Collection location: Boston Bar, Fraser Regional District, British Columbia, Canada [Click for map]
1.5 × 30cm branch Prunus emarginata above Fraser River which has 15 or 16 species of lichen
Several tiny patches of this on the stick. Leave it as R. species (at least for now).
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Totally agree with you. I prefer KOH like you, of course, because it helps separate tissues as well as “clearing” bubbles, etc. But I remember reading in Sonoran Flora (I think) that for Rinodina… yes that’s it… “Spores are best observed in Lugol’s Iodine solution and freeing them from their asci by applying pressure to the cover slip.” KOH will cause things to swell and deform. I guess Lugol’s avoids this problem. My experience (I collected a whole bunch of Rinodina one day in southern California and worked through them all very carefully) was that it did, indeed, work very well, so long as you kept the solution somewhat dilute (I keep my Lugol’s undiluted, so I would just carefully add just the barest fraction of a drop at the edge of the cover slip).
Caloplaca you’re supposed to “cook” for a second in a bunsen burner(!), right? Really?? Who thought that was a good idea!
I also totally agree with you about gathering a diverse collection of specimens and doing a detailed study. My collection from southern California turned out to be very species poor, so of limited use: just helped me get a handle on variation within those species. (Not a bad thing, mind you! But terrible for getting experience with the more exotic spore types, all of which were absent.) Some day…
Working on a Caloplaca recently (which I’ll post) the spores looked 1-septate or biguttulate rather than polarilocular in H2O. In KOH they “became” polarilocular. I hadn’t noticed that before.
Thanks Jason. Previously I’ve looked at a few species of Rinodina, including R. gennarii, which is very common on rock and less so on wood (near salt water) around here, and R. archaea (I think), which is quite common (as very small individuals) on dead Arbutus menziesii branches around here (http://www.flickr.com/...). (Maybe I’ll post all my Rinodina specimens, identified and not identified, on MO.) This specimen from B.C. was very small and my thought was to leave it as R. species. I was thinking that it would be best for me eventually to work seriously on all my specimens of Rinodina at once in a concerted effort to become familiar with the genus. So I’ll postpone a discussion here of ascospore types, (although your suggestions and questions remain interesting and valuable to me). To answer one question though, these were all in 10% KOH. If I recall correctly, Sheard says one must examine spore maturation in water. (For ascospore types prior to Sheard’s 2010 monograph I had been using Mayrhofer & Moberg in Nordic Lichen Flora 2 and Giavarini et al. in Lichens of Great Britain and Ireland.)
(Hope I will be able to see some of your more recent photos of South America. Looked on your other sites.)
Great spore shots! Seems to show early development of septum (type A development), clearly angular lumina, retaining the apical thickening even in the brown overmature spore. Were these shots mounted in just water?
Created: 2013-03-12 11:23:52 PDT (-0700)
Last modified: 2013-03-12 11:34:40 PDT (-0700)
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