Observation 135062: Arcangeliella Cavara
When: 2013-05-31

Notes: Small red capped,short stiped fungi growing on wood. the fungi was remarkably firm to feel and easily removed from the base of a dead stump. The fungi may have been under water during heavy rain in the gorge.

Proposed Names

28% (1)
Recognized by sight
43% (3)
Recognized by sight
31% (2)
Recognized by sight
62% (2)
Eye3 Eyes3
Recognized by sight

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
As for naming . . .
By: Roy Halling (royh)
2013-06-23 11:52:47 PDT (-0700)

I’d go with what Debbie thought, and lean toward one of those sequestrate/secotioid Lactarius species (aka Arcangeliella etc.).

Adding Melzer’s . . .
By: Roy Halling (royh)
2013-06-23 11:45:01 PDT (-0700)

Good on ya Ian in exploring the microscopics of macrofungi! A couple of tricks I learned: (1) if you started out with a water mount, and placed a cover glass on and then wanted to go to Melzer’s, herre’s what I do . . . Do not remove the cover glass, but place Melzer’s at the edge of the cover glass and use a piece of tissue to wick the Melzer’s under the cover glass. Add more Melzer’s until it has permeated the tissue you’re looking at. (2) When viewing a Melzer’s mount at say, 40x or 100x and you are not sure if the spores are amyloid, here’s a tip . . . open the diaphragm of the substage condenser to a wide open position (equivalent to a wide open f-stop on your camera). If there is any tissue or spore that is amyloid, it will be blue-black (or gray in some Mycenas), and the rest will be the color of iodine. Hope this helps.

Last Image loaded – Melzers Staining

When I lifted the top slide and added the Melzers stain the small (pore) ? circles scattered with the solution. The results show some small black circles plus a yellow stainig cell of some description. Not sure what I have here. Help.!!

New Microscope image Comments please

I decided that the previous images were not what I expected and cut another secion of the pileus and placed it on a wet slide. After adjusting the condenser in conjunction with the fine control and brightness slider, I found these small rings in the area immediately away from the physicl body of the specimen. At first I thought they were circles of water, but then noticed the similar markings in some of the circles. Could these be spores that have come away from the specimen when I added the water and the top slide. I did press the top slide to try and remove the water bubbles. I will do a melzers stain now to see what occurs.

IF these are members of the Russulales (Russula, Lactarius)…
By: Debbie Viess (amanitarita)
2013-06-22 08:14:45 PDT (-0700)

then the use of Meltzers, as we discussed in earlier emails, would show you amyloid (blue), ornamented spores. But mount first in water, then stain.

Way to pick a cool micro starter species! ;)

wow Ian, not quite sure what we are seeing here…
By: Debbie Viess (amanitarita)
2013-06-22 08:11:03 PDT (-0700)

sphaerocysts? bizarre spores?

do your images look sharper under the scope or the same as what you show here in your micrograph?

did it have any latex/milk when freshly gathered?

It reminds me a little bit of our secotioid Lactarius sp., like these Arcangeliellas here:

http://mushroomobserver.org/image/show_image/205027?obs=89482&q=1K9Br

Sharper images would help a lot.

Enjoy that learning curve! It is great that you are diving so deeply into the OZ fungi, esp. when you are surrounded by some of the most spectacular wildlife in the world. :)

Three cheers for the Kundabung Kid!

Ian
By: Danny Newman (myxomop)
2013-06-22 00:59:18 PDT (-0700)

If this is indeed your first crack at microscopy, you have already leaped over a long stretch of the learning curve. The problems you described in your email to me about variation in what’s seen through the eyepieces versus the trinocular port are common. Your scope lacks parfocality, either due to the limitations of your components or the need for some minor adjustment. Since I, too, rank low on the microscopy totem pole, I’d recommend heading over to one or both of the following fora:

http://tech.groups.yahoo.com/group/Microscope/
http://www.photomacrography.net/forum/

and creating a post with:

1. your microscope’s specs (make, model, objective magnifications, list of components, etc.)

2. these five images and a description of their subject matter

3. a list of whatever difficulties you’re having (parfocality, clarity, sharpness, etc.)

The membership of one or both sites will help you fine tune your technique far better than I can, as will a handful of other Mushroom Observers, particularly Linas (http://mushroomobserver.org/observer/show_user/4096).

All in all, this is a great start. I’m very excited to finally get to see the previously invisible features of your otherworldly Australian fungi.

Scope Images

This fungi did not have normal gills in my opinion, and as Debbie had noted "this one has a secotioid look to it… (The area had been flooded previously) I am not sure if the specimen is flood damaged or is in its natural state. This is my first attempt at providing enlarged images with a Scope. Any and all comments would be appreciated. The specimen was a dried specimen and water was used on the slide. Some noise and detail adjustments have been made in PS. There are several pecularities in the image that I do not yet understand.
The 5 loaded images are of the same specimen.

this one has a secotioid look to it…
By: Debbie Viess (amanitarita)
2013-06-19 09:18:17 PDT (-0700)

are you ready to throw a gill under your scope yet?

all you need is a drop of water, a slide and a slide cover.

pull one gill off with a tweezers, place on SMALL drop of water on your slide, cover with coverslip, then take a pencil eraser and squish it gently.

start at low power, then work your way up.

look for good examples of spore shapes and color and take a photo thru your scope lens!

voila! micrograph!

Walt

This find was new to me. I kept three specimens for drying, and will have specimen available if requested.

Created: 2013-05-31 04:59:39 PDT (-0700)
Last modified: 2013-06-25 04:57:08 PDT (-0700)
Viewed: 126 times, last viewed: 2016-10-24 05:46:45 PDT (-0700)
Show Log