Observation 136028: Panaeolus fimicola (Pers.) Gillet
When: 2013-06-10
Project: Panaeolus
No herbarium specimen

Notes: Found in a fertilized lawn with Panaeolina foenisecii.


Edit: CCB 08/08/13
ITS BLAST 97% Panaeolus retirugus (China?)
598/604 (99%) ITS sequence similarity with Panaeolus (Observation 137667)

Images

336397
336398
Next to P. foenisceii for comparison.
336478
Immature spores are olivaceous, some mature spores appear to be finely verrucose. 1-3 oil droplets can be present.
336760
Panaeolina front/left for reference.
349893
Copyright © 2013 Christine Braaten (wintersbefore)
Basidia were four spored; KOH; 100-x
349894
Copyright © 2013 Christine Braaten (wintersbefore)
Basidia were four spored; KOH; 100-x
349895
Copyright © 2013 Christine Braaten (wintersbefore)
Basidia were four spored; KOH; 100-x
349896
Copyright © 2013 Christine Braaten (wintersbefore)
clamp; KOH; 100x
349897
Copyright © 2013 Christine Braaten (wintersbefore)
Pilepellis; KOH; 100x
349898
Copyright © 2013 Christine Braaten (wintersbefore)
Pilepellis; KOH; 100x
349899
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia; KOH; 40x
349900
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia; KOH; 40x
349901
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia; 100x; KOH
349902
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia; 100x; KOH
349903
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia; 100x; KOH
349904
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia; 100x; KOH
349905
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia, 100x, KOH
349906
Copyright © 2013 Christine Braaten (wintersbefore)
Sulphidia, 100x, KOH
349911
Copyright © 2013 Christine Braaten (wintersbefore)
Stipe; 100x; KOH
349912
Copyright © 2013 Christine Braaten (wintersbefore)
Stipe; 100x; KOH

Proposed Names

60% (2)
Recognized by sight
Based on microscopic features: Spores look smooth, also I don’t think P. olivaceus has an oblique germ pore like this, Panaeolus cinctulus and P. fimicola do.

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Im working
By: Caleb Brown (Caleb Brown)
2014-02-09 22:01:49 CST (-0500)

On an observation similar to this in that they have the same brownly pigmented cells. I will check in water =)

edit:
I just checked in water and they are still pigmented a golden color

Rocky
By: Byrain
2014-02-09 18:01:18 CST (-0500)

When I saw them, they didn’t seem to be restricted to either the face or edge of the gill & many were deeply embedded in the gills leaving them hard to document.

They are likely
By: Rocky Houghtby
2014-02-09 17:58:15 CST (-0500)

Gloeocystidia of some nature. For instance, Phaeocystidia are versiform, pigmented and may have granular contents. Determining where the cell originates and it’s amyloidoty would help to describe any terminal cell.

Another possibility would be cystidioles, malformed or developing cystidia.

I disagree
By: Byrain
2014-02-09 16:32:31 CST (-0500)

I don’t think they are basidioles or basidia, basidioles & basidia look different in Panaeolus, not so funky/irregular.

Hmmm.
By: Caleb Brown (Caleb Brown)
2014-02-09 16:12:33 CST (-0500)

Your sulphidia is likely pigmented basidioles and basidia, I have yet to see anything that resembles pigmented or reflective cystidia in my observations

I saw that.
By: Byrain
2013-08-13 17:15:15 CDT (-0400)

I don’t think Christine’s photos look much like that either, however they do look much like what Workman has been calling sulphidia, so I will go with that. :)

https://sporeworks.com/photo_gallery.php?cid=6
https://sporeworks.com/photo_gallery.php?pid=13
https://sporeworks.com/photo_gallery.php?pid=24

Compare “sulphidia” to the morphology of c, Abb. 53, p 87.
By: Stephen (Ιερονυμοσ)
2013-08-13 16:17:13 CDT (-0400)

Basidia in Gerhardt 75349 are much more elongated than the spherical sulphidia noted by Gerhardt. though the “sulphidia” in MO 136028 appear smooth.

Looking at the illustration Byrain referenced, Christine’s classic definition of sulphidia proves true as well.

sulphidia
By: Christine Braaten (wintersbefore)
2013-08-12 19:59:55 CDT (-0400)

I don’t think these are pigmented basidioles, I have Gerhardt’s line drawings but I am not certain. I would really like to get to the bottom of this.

Sulphidia?
By: Byrain
2013-08-12 15:02:55 CDT (-0400)

Are you sure they aren’t just colored basidioles? His illustrations don’t look much like these and he does seem to illustrate the refractive part…

Edit: Christine, I revised my opinion, these “sulphidia” look nothing like the sulphidia Gerhardt illustrated in Abb. 54, pg.88, I think you just have basidioles here which would correspond with the colored basidia you have been taking pictures of.

Christian
By: Christine Braaten (wintersbefore)
2013-07-16 20:18:12 CDT (-0400)

Yes, I think there is a problem with the translation, from somewhere I had gotten the impression that they were supposed to be “refractive” but that is clearly not the case with these, maybe that is the case at least some of the time, but I will really need to post some pictures of sulphidia in sulfovanillin.

Christine
By: Christian (Christian Schwarz)
2013-07-16 20:12:06 CDT (-0400)

I’ve encountered problems of sulphidia before (description, definition, recognition). It seems like you have much more experience with them now. I wonder if I could convince you to write a little summary of what you’ve found regarding their definition and recognition, and post it here. I think it would be a big help for people learning microscopy, and to make sure that more experienced microscope workers are interpreting it consistently.

Feel free not to do this, but thanks in advance if you do decide to share!

Panaeolus fimicola
By: Christine Braaten (wintersbefore)
2013-07-16 20:00:11 CDT (-0400)

I think this one is a good example of Panaeolus fimicola:

- Spores within the described range (9) 11-14 (15) x 7-8 (9) x 6-8 microns, these were 13.35 × 9.39 Q=1.42 a little wider.
- Spores are smooth
- germ pore distinctly oblique
- Sulphidia present, what I thought were sulphidia in previous observations were not, these are clearly the sulphidia I’ve been looking for (finally!)


Edit: Spore measurements
Avg 13.35 x 9.39 microns Q=1.42, n=25

Max 16.15, 11.24, 1.62
Avg 13.35, 9.39, 1.42
SD 1.04, 0.63, 0.10
Min 11.42, 8.21, 1.20

13.54 x 9.32
13.44 x 9.15
13.55 x 8.39
14.31 x 9.22
13.51 x 9.50
14.15 x 9.21
12.54 x 9.57
13.79 x 8.97
11.76 x 9.80
12.34 x 9.49
13.36 x 9.31
11.42 x 8.84
12.49 x 9.52
16.15 x 11.24
11.65 x 8.21
13.81 x 9.31
12.54 x 9.09
13.33 x 9.55
13.92 x 10.20
13.81 x 8.51
14.22 x 10.21
12.93 x 9.35
14.48 x 9.96
13.38 x 9.54

Uhhm
By: Byrain
2013-06-10 13:56:00 CDT (-0400)

Skilled mycologists (More so than me) have difficulties identifying these to species macroscopically, how do you do it so easily?

Created: 2013-06-10 12:56:42 CDT (-0400)
Last modified: 2014-02-09 16:38:52 CST (-0500)
Viewed: 949 times, last viewed: 2016-11-27 18:42:34 CST (-0500)
Show Log