Observation 136107: Panaeolus (Fr.) Quél.
When: 2013-06-11
Project: Panaeolus
Herbarium specimen reported

Notes: Growing in well fertilized grass about 50 -100 ft from obs 136028.

Images

336959
1.
336675
2.
336676
3.
336680
4.
336960
5.
348583
Copyright © 2013 Christine Braaten (wintersbefore)
6. Basidia, both two and four spored forms, KOH, 100x
348584
Copyright © 2013 Christine Braaten (wintersbefore)
7. Basidia, both two and four spored forms, KOH, 100x
348585
Copyright © 2013 Christine Braaten (wintersbefore)
8. Basidia, both two and four spored forms, KOH, 100x
348586
Copyright © 2013 Christine Braaten (wintersbefore)
9. Basidia, both two and four spored forms, KOH, 100x
348587
Copyright © 2013 Christine Braaten (wintersbefore)
10. Basidia, both two and four spored forms, KOH, 100x
348588
Copyright © 2013 Christine Braaten (wintersbefore)
11. Basidia, both two and four spored forms, KOH, 100x
348589
Copyright © 2013 Christine Braaten (wintersbefore)
12. Basidia, both two and four spored forms, KOH, 100x
348591
Copyright © 2013 Christine Braaten (wintersbefore)
13. Cheilocystidia, KOH, 100x
348592
Copyright © 2013 Christine Braaten (wintersbefore)
14. Cheilocystidia, KOH, 100x
348593
Copyright © 2013 Christine Braaten (wintersbefore)
15. Clamp, KOH, 100x
348595
Copyright © 2013 Christine Braaten (wintersbefore)
16. Pileipellis, KOH, 100x
348596
Copyright © 2013 Christine Braaten (wintersbefore)
17. Pileipellis, KOH, 100x
348597
Copyright © 2013 Christine Braaten (wintersbefore)
18. Pileipellis, KOH, 100x
348598
Copyright © 2013 Christine Braaten (wintersbefore)
19. Spores with oblique germ pore, KOH, 100x
348599
Copyright © 2013 Christine Braaten (wintersbefore)
20. Spores with oblique germ pore, KOH, 100x
348600
Copyright © 2013 Christine Braaten (wintersbefore)
21. Spores with oblique germ pore, KOH, 100x
348601
Copyright © 2013 Christine Braaten (wintersbefore)
22. Spores with oblique germ pore, KOH, 100x
348602
Copyright © 2013 Christine Braaten (wintersbefore)
23. stipe, KOH, 100x
356899
Copyright © 2013 Christine Braaten (wintersbefore)
24. Sulfovanillin mount, I believe the small brownish irregularly shaped/clavate structures are sulphidia.
356900
Copyright © 2013 Christine Braaten (wintersbefore)
25. Sulfovanillin mount, I believe the small brownish irregularly shaped/clavate structures are sulphidia.
356901
Copyright © 2013 Christine Braaten (wintersbefore)
26. Sulfovanillin mount, I believe the small brownish irregularly shaped/clavate structures are sulphidia.
356902
Copyright © 2013 Christine Braaten (wintersbefore)
27. Sulfovanillin mount, I believe the small brownish irregularly shaped/clavate structures are sulphidia.
356904
Copyright © 2013 Christine Braaten (wintersbefore)
28. Sulfovanillin mount, I believe the small brownish irregularly shaped/clavate structures are sulphidia.

Proposed Names

22% (3)
Recognized by sight
63% (4)
Eye3 Eyes3
Recognized by sight: These appear to be the same: http://mushroomobserver.org/137667?q=1MiFv
both from eastern US in grass, both macroscopically and microscopically very similar (colored basidia, spore size, shape, and color).
17% (4)
Eye3
Recognized by sight
Used references
Based on microscopic features

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
cheilocystidia
By: Christine Braaten (wintersbefore)
2013-08-12 20:35:51 CDT (-0400)

Yes, the few that I did measure would fall into those quoted for fimicola. I have labeled the micrographs so that they can be referenced easily. I believe there are several spores visible that could be described as having an oblique germ pore (Img 12, center; Img 20, second spore from top; Img 21, 3rd from right). There is a scale bar in img 13 and img 14 has a length measurement, but the measurements for cheilocystidia that I have in my notes are 29.72 × 9.36; 21.64 × 6.98; 25.05 × 7.11.

Christine
By: Byrain
2013-08-12 15:50:37 CDT (-0400)

Did you measure cheilocystidia for any of these observations? Gerhardt records a clear difference in cheilocystidia size for P. fimicola and P. cinctulus. Respectively, “25-32 × 6-8 µm” & “30-50 × 8-12 µm”, it could be helpful.

Uhh
By: Byrain
2013-08-12 15:08:05 CDT (-0400)

I looked at all the observations, none of the spores look very oblique excluding obs 136028 which is also the only one that has sulphidia, which kind of look more like basidioles then gerhardt’s illustrations of sulphidia.

Oblique germ pore and ITS sequence for 137667 …
By: Stephen (Ιερονυμοσ)
2013-08-12 13:35:10 CDT (-0400)

Based on spore similarities between both observations obs 137667 and obs 136107 with obs 136028 and the ITS nucleotide sequence for 137667 in comparison with the ITS sequence for 136028, I think it is quite possible these could be “pseudosecotioid” P. fimicola. To quote Chrisitne,

“598/604 (99%) ITS sequence similarity with P. fimicola (Observation 136028).”

“Cap color is variable and eventually lightens to flesh colored in fimicola but is initially described as very dark. But spores are smooth and close to the described range of P. fimicola, slightly wider (11-14 × 7-8); some other things it has in common with fimicola are the noticeably transparent spores and spores that are “significantly brighter than papilionaceus,” and mostly because germ pore very distinctly and strongly oblique. A small cinctulus would lack sulphidia and also have an oblique germ pore within this size range but the spores were much lighter than cinctulus spores.”

Does this have
By: Byrain
2013-08-12 11:54:37 CDT (-0400)

Spores with an oblique germ pore? (not the only Panaeolus that has them) And does this have sulphidia? I don’t see either in the micrographs,i think we should hold off on calling anything P. fimicola without these.

Notes …
By: Stephen (Ιερονυμοσ)
2013-07-19 19:07:35 CDT (-0400)

I never found these in the direct vicinity of the other Panaeolus, but both observations did grow in sporadic little gregarious patches. The farthest “Panaeolopsis” was found several hundred feet away while some as close as 50. The farthest one was growing alone and was not photographed.

collection notes
By: Christine Braaten (wintersbefore)
2013-07-19 17:46:32 CDT (-0400)

I just read in the collection notes above that this observation was found less than 100 feet from observation 136028 which is particularly interesting because they are very similar microscopically, I think if I look again for sulphidia, I will find them!

Panaeolus/Panaeolopsis
By: Christine Braaten (wintersbefore)
2013-07-13 11:55:27 CDT (-0400)

Whether this aligns well with any of the four described species of Panaeolopsis, morphology, other than wilted appearance/incompletely expanded pileus suggests that these belong within/are very closely realated to Panaeolus. It does not seem clearly a match well with a described agaricoid form; it differs from fimicola in possibly not having sulphidia (they may be there, perhaps if I mount in Sulfo-vanillin they will be more apparent), also differs from fimicola by a much longer stipe than is described for fimicola (25-50 × 1.5-3 mm) these look closer to 4-8 cm. Cap color is variable and eventually lightens to flesh colored in fimicola but is initially described as very dark. But spores are smooth and close to the described range of P. fimicola, slightly wider (11-14 × 7-8); some other things it has in common with fimicola are the noticeably transparent spores and spores that are “significantly brighter than papilionaceus,” and mostly because germ pore very distinctly and strongly oblique. A small cinctulus would lack sulphidia and also have an oblique germ pore within this size range but the spores were much lighter than cinctulus spores.


Spore average: 13.98 x 8.79 microns; Q= 1.60

Spore measurements:

16.05 x 9.67
13.53 x 8.70
14.53 x 9.31
14.06 x 7.96
12.76 x 8.11
13.10 x 7.22
14.66 x 10.06
12.58 x 8.27
14.23 x 8.32
14.54 x 10.26
14.85 x 10.32
13.05 x 8.68
15.36 x 10.20
14.07 x 8.04
13.98 x 8.78
13.21 x 8.77
14.88 x 9.40
15.02 x 8.45
13.05 x 7.74
13.07 x 8.47
13.05 x 7.80

max 16.05 x 10.32; 1.81
avg 13.98 x 8.79; 1.60
sd 0.96 x 0.91; 0.12
min 12.58 x 7.22; 1.42

Created: 2013-06-11 10:33:28 CDT (-0400)
Last modified: 2014-06-10 13:29:48 CDT (-0400)
Viewed: 311 times, last viewed: 2016-10-23 22:41:58 CDT (-0400)
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