Observation 138760: Agaricales sensu lato
When: 2013-07-06

Notes: Two specimens found only in one section of the forest surveyed. Pileus and Stipe both Creamy white in colour.
The extra wide and open style of the gills presents towards Russula to me. Unfortunately I cannot find a similar reference image to name this specimen. I have included some scope images hoping that they may help with the ID.(Spore)
From my valuation I would say the spore was Brown. Under higher magnification I found longer thinner cells? Not sure about them. Images loaded.
I also took a high magnification image of one of the spore. Any comments or information to help me in the future would be great. I do not have anyone to fall back on during identification to guide me. This Fungi seems to have much wider spaced gills than anything I have seen so far. The fungi was also delicate although quite big. This may have ben caused by our recent and ongoing rain. Ity was growing in the substrate. Specimen kept.

Images

345509
345510
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Single spore High magnification. (not oil immersed)

Proposed Names

28% (1)
Recognized by sight
62% (2)
Eye3 Eyes3
Recognized by sight

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Ian
By: Danny Newman (myxomop)
2013-07-08 03:16:56 PDT (-0700)

First of all, these spores may very well belong to this mushroom, but a spore print would help support that notion, as it looks to my eye a bit more likely to be light spored than dark. Finding a photographing spores inside asci/attached to basidia is the ultimate confirmation.

I believe distilled water is preferred if making water mounts, which you should be able to buy at most any major grocer. Otherwise, you can use KOH (applying ethanol first and waiting for it to evaporate, especially in the case of dried material).

I would only use lens cleaning solution and lens paper to clean objectives, as ethanol may remove special coatings on the glass (which may or may not actually be present on your specific objectives). Both are available at any photo supply store.

Immersion oil viscosity is, if I’m not mistaken, largely a matter of personal preference. I’m fondest of the more viscous of all possible options, as it doesn’t move around as much on the slide. I’m sure there are optical considerations to be made, but I’m afraid I don’t know enough to offer sound advice. Alan would be a good person to consult there.

I have moved. You should have my new address, so feel free to send package #2. There should be no post office problems this time around.

Scope Images deleted.

Danny, I have deleted the first four Scope images that I loaded to this file. After going back to starters, I realised that something was wrong esp. from your comments. I scoped a clean slide with only water and Bingo, bacteria cells popped up immediately. So you were right on the ball. They certainly did not belong to the fungi. I was unable so far to repeat the other images (left on the file,), but will work on a result hopefully soon. Do you think they are spores of this fungi? I have left the images for comment.

Scope Images

Danny, Thanks for the reply.
I wasn’t able to get a physical spore print although I tried. I do not have any Immersion oil as I wasn’t sure which mix or grade to try and get. I may be able to buy some here in OZ if you can give me the ordering details. Do you use Ethanol for cleaning objectives?
I will redo the scope tests to see if they are different. I will make sure I get a clean start, as you seem to think the spores are foriegn to the fungi.
Also possibly the town water may have miscellaneous bacteria floating around in it.
What is the best alternative to water for a wet slide apart from Immersion oil?
Have you moved yet. The CD I have for you I am sure you will appreciate it. I found it to be extremely well presented.
Thinking about the find, Have you any idea on the ID of this Fungi. The gill structure looks a bit like Marasmius, (Fairy Ring), but this fungi is not to my knowledge found in the Swans habitat. It is mainly found in domestic lawns. But the Gill physical habit is similar. I will post the new scope results as soon as I complete the tests.

Ian
By: Danny Newman (myxomop)
2013-07-06 09:35:34 PDT (-0700)

did you spore print this to comfirm that the spores you saw under the scope indeed belong to this mushroom? Are you in need of immersion oil?

Created: 2013-07-05 21:09:32 PDT (-0700)
Last modified: 2013-07-25 17:22:08 PDT (-0700)
Viewed: 41 times, last viewed: 2016-10-23 00:32:54 PDT (-0700)
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