Observation 139474: Amanita “sp-amerirubescens02” Tulloss cryptonom. temp.
When: 2013-07-10
Herbarium specimen reported

Proposed Names

ret
91% (2)
Eye3 Eyes3
Based on chemical features: nrITS and nrLSU sequences both support this determination.

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Wow
By: Eric Smith (esmith)
2014-07-23 17:53:56 PDT (-0700)

That’s amazing! So similar, yet so different. Thanks for sharing, Rod!

Your comment about the two taxa appearing in the same place…
By: R. E. Tulloss (ret)
2014-07-22 19:15:02 PDT (-0700)

made me wonder about the genetic distance between the two species. Our software can take all the …02 barcodes and the two …07 barcodes and match them all to each other pairwise. A simple matrix of distances can be generated based on percent of characters that differ (checked position by position in the string of characters that make up a sequence.

The two ..07 sequence have no difference (0% is the distance).

The eleven ..02 sequences have differences ranging from 0 to 0.6%.

The differences between sequences of ..07 and sequences of ..02 range from 5.2 – 6.0%. Roughly speaking there is ten or more times as much difference between the two species as there is between “individuals” of the species with the greatest apparent intraspecies gene variation.

You made me curious; so I thought you’d like to know the answer I came up with.

Very best,

Rod

Thanks Rocky
By: Eric Smith (esmith)
2014-07-22 18:37:03 PDT (-0700)

I appreciate the kind words. It’s all an experiment. This one gives the eyes a lot of places to go…

And thanks again Rod, for the generous, diligent feedback. It is very much appreciated by us collectors!

Interestingly, this collection was made very close to the collection from observation 147255, Amanita “sp-amerirubescens07”.

Eric
By: Rocky Houghtby
2014-07-21 16:22:11 PDT (-0700)

That is some damn fine photography, bud!

We obtained DNA (ITS and LSU sequences) from this material, Eric.
By: R. E. Tulloss (ret)
2014-07-21 15:16:36 PDT (-0700)

The sequences indicate that this collections belongs to Amanita sp-amerirubescens02.

Very best,

Rod

Thanks Eric,
By: groundhog
2014-04-18 11:17:01 PDT (-0700)

This material has been recieved and accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing.
-Naomi

Created: 2013-07-10 20:00:46 PDT (-0700)
Last modified: 2014-07-21 17:35:44 PDT (-0700)
Viewed: 94 times, last viewed: 2016-07-28 22:59:44 PDT (-0700)
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