Observation 140397: Amanita brunnescens G.F. Atk.

On moss covered ground under balsam fir (Abies balsamea) and hemlock (Tsuga cadensis) in mixed woods including white pine (Pinus strobus), gray birch (Betula populifolia) and white cedar (Thuja occidentalis). Cap 80 mm. wide, pale brown (no red tones), paler at margin, buff colored patches, flesh of cap 4 mm. thick above stipe. Gills white to pale cream, close. Stem white bruising slowly red, 112 mm. from bottom of cap flesh to top of bulb, 10 mm wide at mid-height, whitish partial veil on upper stipe, volval remnants on lower stipe. Bulb white staining red, 20 mm. long x 23 mm. wide at broadest point. Dried specimen will be sent to Rod Tulloss for further study of rubescent taxa in eastern US, see MO 140191 for his comments.

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This material has been sequenced. … EDITED
By: R. E. Tulloss (ret)
2014-03-22 12:02:45 EDT (-0400)

This material’s ITS and LSU gene sequences are definitively those of A. brunnescens. Also, it is interesting that Dr. Hughes was able to distinguish two forms (slightly differing) of the ITS locus sequence in this material.

This collection will be added to the techtab of the following page today:


Very best,


Hi Teresa,
By: groundhog
2013-08-01 10:10:08 EDT (-0400)

This material has been received, accessioned to Rod’s herbarium, and scheduled for sequencing.

Hello, Teresa.
By: R. E. Tulloss (ret)
2013-07-22 12:45:24 EDT (-0400)

Thanks again for your generosity.


Hi Rod,
By: Terri Clements/Donna Fulton (pinonbistro)
2013-07-22 08:24:18 EDT (-0400)

This was mailed to you today with two others.



Thanks, again.
By: R. E. Tulloss (ret)
2013-07-19 12:56:38 EDT (-0400)

Actually you get bonus points because, since the stem didn’t bend, you must have had the mushroom vertical overnight.

We’re delighted that you’re planning to send this material.

Very best,


Thank you Rod
By: Terri Clements/Donna Fulton (pinonbistro)
2013-07-19 12:36:00 EDT (-0400)

for your comment. I wanted to make you aware of the following: This specimen was collected because it was in the best condition of the group (see MO 140409) but the cap was not fully opened. As it was collected too late in the day to deal with I put it in a cool area overnight and it looked like this the next morning.



Thank you, Teresa.
By: R. E. Tulloss (ret)
2013-07-19 11:43:22 EDT (-0400)

You documented the bulb very thoroughly. Thanks for that. Your habitat is also of interest to our project. What we’re finding is that there is the genetic picture is more complex every time we get a new set of sequences. We clearly need more material from more sites.

Different sites in a county, different ecosystems, different counties in a state or province, different states, different provinces, different countries, etc.

Right now our “amerirubescens” sequences come from a very limited number of states/provinces: Connecticut, Florida, Missouri, New Jersey, Newfoundland, Pennsylvania, and Texas. Remember (folks who have sent samples from other states/provinces) not every sample yields DNA, not every sample has been processed to see if DNA can be extracted, and we haven’t sampled 200 collections or more that we know we want to sample.

Thanks to everybody for keeping with the project. We will have a hiatus in sequencing activity in the fall semester of the coming academic year.

Very best,


Created: 2013-07-19 10:11:23 EDT (-0400)
Last modified: 2014-03-22 14:39:54 EDT (-0400)
Viewed: 171 times, last viewed: 2018-02-21 23:45:10 EST (-0500)
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