Observation 140409: Amanita brunnescens G.F. Atk.
When: 2013-07-18
No herbarium specimen

Notes: On moss covered ground under hemlock and balsam fir in mixed woods that includes white pine and gray birch. These old and insect ridden FB’s were about a meter from MO 140397 and probably the same thing. White spore deposit.

DNA sequencing on obs 140397 shows it to be A. brunnescens—this is likely older version of same species.

Proposed Names

-28% (1)
Recognized by sight: Stem bruising red; no cleft bulb

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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From Rod Tulloss
By: Terri Clements/Donna Fulton (pinonbistro)
2013-07-19 13:39:25 PDT (-0700)

in response to my questions on sampling:

All good questions.

Old and buggy is not so useful; you are right.

Incompletely open are not so useful for spores and the anatomy of the gills;
however, they can be very valuable for the layering of the skin of the cap and
any interconnection between the volva on the cap and the skin of the cap. The
surface of the cap in many species of Amanita progressively gelatinizes with
time until the mushroom decays; so it is very important to understand how it
changes. The gelatinizes allows the volval patches to slip and slide and get
washed off in the rain. In cases of very powdery volvas, they come apart very
easily and sometimes everything still works without gelatinization. The latter
situation is the case with Amanita farinosa, which holds onto the veil for quite
a long time. The hyphae that have been connecting the volva and the skin
(pileipellis) of the cap from the beginning of their development in the
primordium (tiny knot that becomes the button) don’t disappear for a long time
and actually bind the volva to the cap.

Amanita seems to try every variation that one can imagine…or maybe my
imagination is limited.

Anyway, the most information from a single specimen comes from a specimen that
has just mature and is capable of producing a good spore print. At this point,
the sample of spores is large and you can get a reasonable idea of the variation
in size and shape. The gills will have significant mature areas to examine with
regard to mature anatomy. The pileipellis is not entirely deteriorated. Happy
days.

Sampling for DNA should work on fairly mature buttons upto, but not including,
old age and decay (too many organisms eating the mushroom at that point…along
with higher potential for breakdown of the mushroom’s DNA.

I hope this helps a bit.

If you find it useful, maybe you could post it on a recent observation of yours
on MO…so more people could see the question and response.

Thanks for asking.

I’ll check your attachments, too.

Very best,

Rod

Dr. R. E. Tulloss
ret@eticomm.net [<= Share DropBox folders to this email only, please]
retamanita@comcast.net

www.amanitaceae.org

Herbarium Amanitarum Rooseveltensis
P. O. Box 57, Roosevelt, NJ 08555-0057, USA

Created: 2013-07-19 09:26:16 PDT (-0700)
Last modified: 2014-03-22 20:09:49 PDT (-0700)
Viewed: 49 times, last viewed: 2016-10-26 00:41:58 PDT (-0700)
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