Observation 141103: Ganoderma oregonense Murrill
When: 2013-07-21
(34.368° -111.004° 2400m)

Notes: Extremely soft and fresh, following about 5 inches of summer monsoon rain in previous 2 weeks. Growing on dead Douglas Fir.

Images

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505499
From a specimen collected on the same stump in July 2014. 50X oil immersion with 3.3X photo eyepiece, ISO 100, 1/2 sec exposure, Nikon 7000.

Proposed Names

25% (3)
Eye3
Recognized by sight
Used references

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Very good observation.
By: MSchink
2015-02-21 15:16:12 CST (-0500)

Yes that is a very good point I have noticed this myself but have not really taken the time to get acquainted with the thickness of the different species.

Hi
By: Erlon (Herbert Baker)
2015-02-21 14:38:48 CST (-0500)

Keep in mind the spore wall thickness may vary between species and may provide important clues for delineation.

Measurements
By: MSchink
2015-02-21 14:10:55 CST (-0500)

I think the discrepancies could be in the way I measure the spores.I chose to measure mine from the inside spore wall, because the outer wall tends to get broken and can lead to varying size. Notice how some spores have a rounded end and others will appear trunicated.

Part of the reason you can find varying measurments in the literature for Ganoderma is because not everybody measures using the same method, I should bhave mentioned my method. They were also mounted in 90% isoproyl alcohol. I have read of some spores swelling in KOH.

This method ends up giving me slightly smaller spore sizes than in the literature for certain species, but usually not anything taxonomically significant.

Looking at my saved records spore sizes these measurements fall between G.tsugae and G.oregonense. (Little larger than the typical tsugae, but smaller than the oregonense) I definitely think this specimen represents an intermediate between the two. I plan on sending a piece for sequencing to see what that can tell us.

Spore wall
By: Erlon (Herbert Baker)
2015-02-21 13:29:47 CST (-0500)

are you measuring the entire spore, or just within the spore wall?

My measurements were:
By: Chris May (ccmaymd)
2015-02-21 13:16:08 CST (-0500)

7.9 [9.4; 10.2] 12.2 × 5.2 [6.9; 7.4] 8.2 µm; Q = 1.2 [1.3; 1.4] 1.6; N = 30; C = 95%; Me = 9.8 × 7.2 µm; Qe = 1.4

I wonder if I’m measuring them properly. New at this!

We are pretty close, I think. Could my marginally larger diameters be attributable to the mounting fluid? I used 5% KOH. If not, then I am probably measuring them slightly differently.

Spore size
By: MSchink
2015-02-20 11:40:39 CST (-0500)

Here are the measurements I got for the spores.

7.5 [8.8 ; 9.4] 10.7 × 5.3 [6.2 ; 6.6] 7.5 µm
Q = 1.2 [1.4 ; 1.5] 1.7 ; N = 30 ; C = 95%
Me = 9.1 × 6.4 µm ; Qe = 1.4

8.36 6.05
7.89 5.44
9.20 6.05
8.50 5.09
9.46 5.89
8.56 6.58
9.54 6.44
8.56 6.22
8.55 6.54
8.07 6.00
9.57 6.47
10.65 6.84
10.74 6.46
10.29 5.98
8.83 5.33
8.78 6.60
7.98 6.81
8.45 6.49
9.40 6.77
9.06 6.20
8.90 5.82
8.48 6.81
9.11 6.26
9.53 7.04
9.09 6.68
11.16 6.87
8.81 6.29
9.68 7.18

So I got this specimen.
By: MSchink
2015-02-14 21:45:54 CST (-0500)

Thank you.

This is very interesting, the spores are small like tsugae, the flesh is much harder than G.oregonense. The pores however are fairly large matching oregonese, about 2-3 per mm compared to about 4 per mm with tsugae. I think this likely represents an intermediate form between the two species.

Created: 2013-07-26 23:03:29 CDT (-0400)
Last modified: 2015-09-14 01:27:35 CDT (-0400)
Viewed: 207 times, last viewed: 2016-10-22 17:03:01 CDT (-0400)
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