Observation 146611: Amanita “sp-OR01” Tulloss crypt. temp.
When: 2013-09-24

Notes: Single specimen growing near Sitka spruce @ ~ 250 ft elevation.
I’ve found a few similar looking Amanitas in this general area before but this one is a little fresher.
Cap was 8.0 cm across and the stipe 18.5 cm high.
Spores were white, not amyloid, and ~ 11.0-14.2(15.1) X 10.1-14.1(14.8) microns,
globose to subglobose(Q average = 1.03).

Proposed Names

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Ron,
By: Noah Siegel (Noah)
2017-02-11 01:40:10 CET (+0100)

Thanks for bringing this to my attention.

I have seen it just north of Big Lagoon; (NS1037), and along the OR, and BC coast. I think the people I was with got collections in BC; I’ll look into that. I have also seen pictures from Cordova that appear to be this species, but I have not collected it there.

Rod, I’ll follow up with privately, as I have sent you a collection of this.

That would be very helpful, Ron.
By: R. E. Tulloss (ret)
2017-02-10 21:56:51 CET (+0100)

Thank you.

Very best,

Rod

Great news Rod….
By: Ron Pastorino (Ronpast)
2017-02-10 21:09:20 CET (+0100)

and I see Noah has an posting for Amanita “sp-OR01” from a little further south in 2013.
I’ll check with him to see if he has a voucher or photos.

Thank goodness I have relatively good way to store incoming data and to
By: R. E. Tulloss (ret)
2017-02-10 19:40:30 CET (+0100)

store the same data separately by probable species. It also helps to have data search features for both the herbarium collections and the DNA sequences. Buying a “no commercial use” copy of a DNA processing package has been very useful. Although there are good free copies that several people will swear by.

Very best,

Rod

The story gets better on the nrLSU side.
By: R. E. Tulloss (ret)
2017-02-10 18:40:01 CET (+0100)

It turns out I have nrLSU sequences over 1300 characters long for all three collections, and these sequence are exact matches. The case for a single species is yet stronger.

Very best,

Rod

Good news.
By: R. E. Tulloss (ret)
2017-02-10 17:35:28 CET (+0100)

I have over 1400 characters of nrLSU from both the present case and 629-3. They are exact (100%) matches. I think we can say that our eyes did not deceive us (this time). The three collections all represent a single species.

Thanks again, Ron. I’m very grateful.

Very best,

Rod

Good stuff and good summary!
By: Ron Pastorino (Ronpast)
2017-02-10 17:32:05 CET (+0100)

Thanks Rod.

I have found the data for RET 417-10 also. It also has a damaged nrITS…
By: R. E. Tulloss (ret)
2017-02-10 16:47:11 CET (+0100)

Sequence. In comparing the 417-10 and 629-3 data for the damaged sequences, I can see the sequences are identical expcept for the “damaged” region, which is 34 characters long if the two sequences are aligned. The species are quite similar genetically.

If readers take a look at this page

http://www.amanitaceae.org?nrITS%20Sublocus%20Termini%20in%20the%20Amanitaceae/... ,

they will see that the “proposed fungal barcode gene” is composed of three parts (ITS1, 5.8S, ITS2) and bounded on the two ends by two other genes (18S or SSU and 28S or LSU). You can check the quality of your data for the “barcode gene” by looking for the nearly constant strings of characters that mark the ends of some of its pieces.

In the “barcode gene” data for 417-10 and 629-3, I can see clearly the strings (subsequences) marking the “right hand” end of 18S (“GGAAGGATCATTA”) and the “left hand” end of 28S (“TTGACCTCAAATCA”). [Note that the presence of the latter substring indicates sp-OR01 does not fall in series Penetratrices.] So we already know something about the species.

So the damaged area falls somewhere within the “barcode gene” proper.

So the next thing to look for is the “left hand” end of 5.8S (which has a fairly constant pattern that is easily recognizable. Less than 20 characters following the “right hand” end of the “damage zone” I find the “left hand” end of 5.8S (in this case ACAACTTTCAACAAT). Now we have nailed the position of the damage zone. It falls near the “right hand” end of the ITS1 part of “barcode”. We can handle that because in many, many amanitas, the ITS2 region, alone, can work like a “barcode.”

Therefore, the data supports 417-10 and 629-3 being very closely related if not the same taxon. The morphology drove us in the same direction all along. The story will continue.

Very best,

Rod

RET 629-3 had DNA extracted. The “barcode” fragments received could…
By: R. E. Tulloss (ret)
2017-02-10 16:08:21 CET (+0100)

not be combined into a complete sequence because of a large central gap in the data. It is consistent with this result that we failed to get data for the “proposed fungal barcode gene” in the present case as well.

I have found a set of notes for 629-3 along with the original data. It looks like I was interrupted when I was working on it. Maybe there is more to be said after I reconstruct what I was doing.

Thanks for the prod.

Very best,

Rod

Rod, I forgot to ask if this new sequence
By: Ron Pastorino (Ronpast)
2017-02-10 02:37:06 CET (+0100)

is close to my older collections from the same general area…http://mushroomobserver.org/4307 which included your RET 629-3 and 417-10?

Rod, thanks for the update.
By: Ron Pastorino (Ronpast)
2017-02-10 01:08:04 CET (+0100)

Maybe this year, I’ll find more.

DNA for this species appears to be unique among all sequenced species of…
By: R. E. Tulloss (ret)
2017-02-09 23:29:29 CET (+0100)

sect. Vaginatae. Nothing that is relatively close genetically is morphologically similar.

The nrLSU gene (which we have) supports the thinking that this could be a new species.

Very best,

Rod

Thanks Rod
By: Ron Pastorino (Ronpast)
2013-10-28 00:37:59 CET (+0100)

Ron

I’m adding all the data updates here:
By: R. E. Tulloss (ret)
2013-10-27 17:34:52 CET (+0100)
Working on this material today.
By: R. E. Tulloss (ret)
2013-10-27 17:29:56 CET (+0100)

One character that might be useful in the field is that there are very few short gills on the cap and these are mostly a little less than half the length of a full-length gill. There are no very short short gills as are often seen in amanitas. The material dried with the gills nicely and distinctly separated so that the short gills can be clearly seen.

Also the gills are distinctly marginate. They may have had an orange margin originally; however, in the dried material, the gills’ margins have become dark brown.

Very best,

Rod

Thanks Ron,
By: groundhog
2013-10-25 20:41:14 CEST (+0200)

This material has been recieved and accessioned into Rod’s herbarium.
-Naomi

Hi Ron,
By: groundhog
2013-10-22 19:38:45 CEST (+0200)

We are going to call this sp-OR01. We will let you know more when we know more.
-Naomi and RET

Thank you, Ron.
By: R. E. Tulloss (ret)
2013-09-27 22:57:13 CEST (+0200)
Rod, the Vaginatae is on its way to
By: Ron Pastorino (Ronpast)
2013-09-27 22:28:31 CEST (+0200)

Herbarium Rooseveltensis Amanitarium.
Ron

If no one has asked for this,…
By: R. E. Tulloss (ret)
2013-09-27 20:26:11 CEST (+0200)

may I take a look?

Very best,

Rod

Created: 2013-09-27 19:02:02 CEST (+0200)
Last modified: 2013-10-22 20:09:36 CEST (+0200)
Viewed: 191 times, last viewed: 2017-06-16 21:29:46 CEST (+0200)
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