Observation 151170: Amanita dulciarii Tulloss nom. prov.
When: 2013-11-03
Herbarium specimen reported

Notes: A single fruiting body found in a close proximity to the collection in MO #151169.
This material is saved for RET.

Proposed Names

ret
-54% (1)
Recognized by sight: See comments.
Based on microscopic features: Spores similar to those on the first collection of this taxon.
Based on chemical features: inamyloid spores

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Thank you for posting, Rod
By: I. G. Safonov (IGSafonov)
2013-12-21 05:12:35 UTC (+0000)

I am glad the identity of this mushroom specimen was unambiguously confirmed by the DNA barcoding method. In light of this discovery, I shouldn’t feel so bad about misidentifying the holotype of A. vulpecula (MO148482) as A. dulciarii.

This immature specimen is a genetic match to dulciarii.
By: R. E. Tulloss (ret)
2013-12-19 20:20:18 UTC (+0000)

We have our first sequences back from the “vulpecula” collections. This one is not vulpecula.

The ITS sequence from this observation is an extremely close match to ITS sequences from typical specimens of A. dulciarii.

Very best,

Rod

Thank you, Igor.
By: R. E. Tulloss (ret)
2013-11-08 18:32:59 UTC (+0000)

I forgot to mention that I also need the width of the (hollow) central cylinder of the stem measured at the same point on the stem at which the stem width is measured.

I much appreciate your willingness to work out these dimension. They greatly increase the value of the dried material.

Very best,

Rod

Measurements
By: I. G. Safonov (IGSafonov)
2013-11-08 18:18:23 UTC (+0000)

Hello, Rod:-

I will provide the measurements for you later this afternoon.

Hello, Igor.
By: R. E. Tulloss (ret)
2013-11-08 13:43:57 UTC (+0000)

Could you help me with the last image (cross section)? In the upper left corner of the picture there are two dots. Could you measure the distance between the dots and either send me the measurement or apply it your self in order the measure these items:

Thickness of cap directly above the stem, width of stem at the middle, width of bulb at the broadest point, length of bulb, length of stipe from the bottom of the cap to the top of the bulb, and breadth of the gill at the broadest point (about 75% of gill length from stem to cap margin). All this will be very helpful to me.

Very best,

Rod

When the sand is removed from the stem base,….
By: R. E. Tulloss (ret)
2013-11-07 22:13:52 UTC (+0000)
this mushroom has the same slender bulb with a raised margin as is seen in Amanita sp-55. So I don’t think that this is A. dulciarii.

The inamyloid spores are very few. The hymenium is almost entirely immature/sterile. There are a few large spores of the approximate size and shape of those of sp-55 floating in a mount of gill sections from immediately adjacent to the stem (the place where sporulation begins). Sections from further away from the stem have no mature basidia at all.

It’s important to get spore prints of this entity before drying…so that we have sufficient maturity of the gills to provide a realistic sample of spore sizes and shapes in order to understand the variation of these quantities.

From the images and the above data, I would suggest calling this material sp-55 rather than dulciariii.

Very best,

Rod

Created: 2013-11-04 19:49:37 UTC (+0000)
Last modified: 2013-12-19 23:12:01 UTC (+0000)
Viewed: 77 times, last viewed: 2016-08-19 16:01:50 UTC (+0000)
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