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|I’d Call It That||3.0||0.00||0|
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LSU costs the same as ITS, about $8. Some entries in GenBank have only LSU, so sometimes there are matches with that gene region where ITS doesn’t show matches. If this is going to be described as new, it is best to get as many gene regions as possible.
The data should arrive tomorrow morning. Should we do LSU as well?
Matches 95% with three different Melanoleuca species. The sequence had a lot of noise towards the end due to enzyme slippage caused by 6 T’s in a row – if you are serious about studying this one I can have them read the reverse strand which will improve data quality of the last 200 bases. The lower case characters are the ones I changed – this was done by viewing the chromatogram with FinchTV and using the close BLAST matches to suggest what the base actually is in the cases of ambiguity. If the close BLAST match found a different base than I had and the chromatogram showed a peak of the suggested base, it was changed. If the chromatogram did not show any sign of the suggested base being present, it was considered a real difference between this sequence and the BLAST matches, and left alone. This sequence is probably over 99% accurate and may be 100% accurate.
Created: 2013-12-10 06:47:26 CET (+0100)
Last modified: 2013-12-10 06:47:34 CET (+0100)
Viewed: 62 times, last viewed: 2017-06-18 00:28:43 CEST (+0200)