Observation 160073: Echinoderma hemisclerum (Berk. & M.A. Curtis) Courtec.
When: 2013-07-13
( 1500m)
No herbarium specimen

Images

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Gill edge 400x
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Gill edge 400x
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Cheilocystidia crush mount 400x
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Cheilocystidia crush mount 400x
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Cheilocystidia crush mount 400x
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Spores and basidia 1000x
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Spores 1000x
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Phylogenetic tree made with ITS sequences shows that this collection is very close to Lepiota aspera and should be in the genus Echinoderma.

Proposed Names

87% (1)
Eye3 Eyes3
Based on chemical features: ITS sequence shows that this is is in a clade with other species which have been moved to Echinoderma

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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By: Alan Rockefeller (Alan Rockefeller)
2014-06-17 04:26:30 BST (+0100)

I found the name L. hemisclera on MO. Alonso has been posting pics of it for a few years, and he took me to his spot where I found these.

Nice work, Alan!
By: Irene Andersson (irenea)
2014-06-16 20:42:50 BST (+0100)

and a very special mushroom. Where did you find the name hemisclera?

Nice record!
By: walt sturgeon (Mycowalt)
2014-06-16 01:47:41 BST (+0100)
ITS Sequence / phylogenetic tree
By: Alan Rockefeller (Alan Rockefeller)
2014-06-16 00:39:36 BST (+0100)

Since this species looks so different from other Lepiotas and there were no L. hemisclera sequences in genbank, I decided to check the DNA to determine its phylogenetic position.

Last week I invited Jacob Pulk to the lab and he did a DNA extraction using a modified version of the NaOH protocol published in Osmundson 2013. I ran a PCR reaction using stemandcell master mix with its1f and its4b primers. I brought two PCR products to Genewiz for sequencing, one with the its1f primer and the other with the its1b primer to get the reverse compliment I generated a consensus sequence with Bioedit and used Mega6 to build a phylogenetic tree which includes my collection. To make the tree I used the maximum likelihood algorithm with the Kimura two parameter model, gamma distributed rates among sites, and gaps treated with the partial deletion algorithm.

The results show that L. hemisclera is very close to Lepiota aspera, which has recently been moved into the genus Echinoderma. Though Index Fungorum shows that Lepiota hemisclera it is currently in Cystolepiota following Pegler 1983, my tree shoes that Echinoderma is the correct genus for this taxon. My findings agree with Régis Courtecuisse’s 2004 paper where he moved L. hemisclera into Echinoderma.

Wow!
By: Erlon (Herbert Baker)
2014-06-15 20:52:24 BST (+0100)

What a beautiful species!

Created: 2014-02-20 21:40:01 GMT (+0000)
Last modified: 2014-06-16 00:37:48 BST (+0100)
Viewed: 97 times, last viewed: 2016-09-20 03:57:02 BST (+0100)
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