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of Cladonia. Cup shaped Lichen ( C. grayi); Finger-like Lichen ( C. ochroclora).
Microscopy can be mysterious. It could definitely be dissolving. Or maybe the rest of the spore is inflating in K. Also, KOH has a different index of refraction than water. Some things are more easily discernible in KOH, others in water, because of this.
Makes chemistry fun. Question for you. On one of Dave W’s obs. he showed apiculi which Kuo stated was best observed in water mounts and not readily seen in KOH. Would the apiculi become soluble in KOH and not be seen? I belive his obs was of a Discina like fungus.
TLC = thin layer chromatography. You get these special permeable TLC plates and put a drop of acetone extract (a fragment of your lichen in a drop of acetone) near the bottom, then place it upright in a jar with the bottom in a tiny bit of one of several exotic (and highly toxic) solvents. As the solvent diffuses upward, different substances travel upward at different rates. When the time is up, you char it or look at it under UV and mark where the various resulting spots appear. There are vast catalogs of all the known lichen substances and how quickly they diffuse in each of the standard solvents. In principle this can be done in your bedroom (you have a fume hood in your bedroom, right?), but not only does it require access to several nasty reagents not available in your neighborhood drug store, but it apparently also requires a great deal of experience to interpret the results reliably. For various reasons I have not invested in the technology yet! :)
By TLC I thought you meant tender loving Care as applied to having a great deal of patience in studying these organisms. What is TLC?
But I don’t have access to TLC, so there’s nothing more I can do with it. NYBG has an extensive collection of lichens from all over Pennsylvania. If you have good collections and are looking for a place for them to live, you might try contacting them.
If you would like a sample I could mail a section of this to you.
The cupped thing is probably C. grayi because I think that’s the species of the C. chlorophaea group which occurs on logs in the east. (In general, one needs TLC to determine that group to species; we’re just taking advantage of where you found it and hoping the folks at NYBG have done their work correctly!)
The un-cupped thing is C. ochrochlora, nice and easy. You can see the smooth corticate sheath near the base. Other subulate species would be either squamulose or granular / powdery right to the base.
Created: 2014-05-22 19:49:54 CDT (-0400)
Last modified: 2017-09-11 18:28:52 CDT (-0400)
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