Observation 167169: Boletales E.-J. Gilbert
When: 2014-06-11
Herbarium specimen reported

Notes: growing in slash pine

Images

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425835
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425837
shiny object is a dime
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cap before picture
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immediate blue/green staining
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spread of the blue green, basically bleached out the spot where the drop was placed
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after stipe, no noted change
425986
before stipe

Proposed Names

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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*Bc i only have chunks
By: Jimmy Craine (doctorghosty)
2014-06-17 23:19:55 EDT (-0400)

*Bc my dehydrator is ronco

I will continue to monitor the spot however
By: Jimmy Craine (doctorghosty)
2014-06-17 23:18:18 EDT (-0400)

I have some dried material, for culinary purposes, do you need a fully intact dried specimen?

Will do, Igor.
By: Dave W (Dave W)
2014-06-14 14:06:42 EDT (-0400)

Now all we need (hopefully!) is one or two substantial rain events to close out this month.

Samples for sequencing?
By: I. G. Safonov (IGSafonov)
2014-06-14 14:00:02 EDT (-0400)

I could probably get ITS sequences for a few samples this year. It would be interesting to run Dave’s bluing and non-bluing B. subcaerulescens specimens as well as Jimmy’s cryptic bolete. As sequences for vouchered specimens of B. subcaerulescens, pinophilus and separans exist in GenBank, chances are that we should be able to get interpretable and meaningful results. Let me know if you are interested in sending me your samples.

Thanks Arleen.
By: Dave W (Dave W)
2014-06-14 13:13:58 EDT (-0400)

There’s one spot in my locality where the completely non-staining subcaerluescens type occurs… just not very often. In the past I had called it “pinophilus” as it matched well with the version found in the NA bolete manual. Seems to require abundant rainfall at just the right time, late June. If I encounter it again, I’ll put aside a few specimens.

And, I really need to remember to buy some ammonia!

David…
By: Arleen Bessette (MsMucor)
2014-06-14 09:18:13 EDT (-0400)

yes, it seems more than likely that your collections are all B. subcaerulescens with variable blueing (vs. staining w/NH4OH)reactions.

Re: Boletus pinophilus, there needs to be more work done on those collections identified as being B. pinophilus. Specifically, there needs to be macrochemical tests done and perhaps more importantly, DNA sequencing.

The European B. pinophilus has been sequenced and compared to the American B. subcaerulescens and they are two distinct species, in two different clades (Molecular phylogenetics of porcini mushrooms – Boletus section Boletus by
Bryn T.M. Dentinger, et al.).

Based on all of this, we are not certain if B. pinophilus occurs in the USA.

Igor -
By: Arleen Bessette (MsMucor)
2014-06-14 09:05:24 EDT (-0400)

After restudying JC’s images from last yr, we now believe that it is not B. separans. Boletus separans, in our opinion, should have more of the pinkish coloration at the apex rather than below it. The age of the specimens makes a huge difference in their appearance.

As far as B. subcaerulescens and B. separans growing together in the same location, who knows?

What I’m wondering…
By: Dave W (Dave W)
2014-06-13 19:18:23 EDT (-0400)

is whether the bolete documented as Boletus pinophilus in Bessette/Roody/Bessette is actually a non-staining variety of B. subcaerulescens. I have IDed collections of subcaerulescens that exhibit variable bluing, some not at all (from the same collection as stainers). And I have collected what keys out well as the B/R/B version of B. pinophilus, non-staining. Both types seem to associate with White Pine in my area.

.
By: Danny Newman (myxomop)
2014-06-13 14:16:06 EDT (-0400)

makes me wish I’d posted photos of the reactions on Observation 147920. they were… ambiguous.

MO136830
By: I. G. Safonov (IGSafonov)
2014-06-12 20:13:30 EDT (-0400)

Hello, Arleen. Thanks for the info on the distribution and associations of B. subcaerulescens.
JC just confirmed (thanks!) that both collections came from the same spot. I believe that you and Alan IDed MO136830 as B. separans (personal communication). Is it possible that both species grow in the same location or …are they actually the same thing? It would be interesting to know what you think…

Igor -
By: Arleen Bessette (MsMucor)
2014-06-12 20:00:01 EDT (-0400)

not certain about vouchered collections. However, Susan & Van Metzler reportredly collect it often in Texas under long-leaf pines and it has also been collected by members of the Gulf States Mycological Society.

Yessir
By: Jimmy Craine (doctorghosty)
2014-06-12 19:55:33 EDT (-0400)

Same spot

Same as MO136830?
By: I. G. Safonov (IGSafonov)
2014-06-12 19:45:52 EDT (-0400)

I am curious, JC, if you found these in the same spot as those of MO13680 from last year (also collected in June)? The identity of those was a bit more elusive.
B. subcaerulescens has been reported to grown under Scots pine and spruce in the northern states. It’s possible it shifted to other hosts in Pinaceae. Has anyone actually collected vouchered samples of B. subcaerulescens in the South?

thank you
By: Jimmy Craine (doctorghosty)
2014-06-12 19:11:28 EDT (-0400)

enjoyed it thoroughly.

This appears to be Boletus subcaerulescens…
By: Arleen Bessette (MsMucor)
2014-06-12 18:57:18 EDT (-0400)

because of the fine reaction you have documented by applying NH4OH (ammonia) to the fruit body! B. subcaerulescens stains the cap bluish-green, then bright orange surrounded by greyish-green, then entirely yellow-orange. Great ID work, James! :)

ammonia test results
By: Jimmy Craine (doctorghosty)
2014-06-12 18:48:12 EDT (-0400)

cap stained blue/green, stipe didn’t stain anything at all. pictures added to observation reflecting those findings.

I’ll do it when I get home from work
By: Jimmy Craine (doctorghosty)
2014-06-12 12:06:13 EDT (-0400)

!

Yes! If you can…
By: Arleen Bessette (MsMucor)
2014-06-12 11:51:32 EDT (-0400)

… please do test with NH4OH and report reaction!!

Can you
By: Noah Siegel (Noah)
2014-06-12 09:58:55 EDT (-0400)

put a drop of ammonia on the cap and stipe and report the reaction?

Created: 2014-06-11 19:25:46 EDT (-0400)
Last modified: 2015-07-17 00:23:43 EDT (-0400)
Viewed: 227 times, last viewed: 2016-10-29 01:32:04 EDT (-0400)
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