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it is very distant from the part of LSU that we are usually able to obtain.
“Old species 17” and “rooseveltensis” (not on the website yet) and other species with average spores ellipspoid are going onto the agenda. We’ll see what we can get.
Sometimes the “proposed fungal bar code” (nrITS) gene appears in multiple copies within one fruiting body (indicating hybridization within a biological species).
This is particularly common in some amanitas. So difficulties in getting a complete and coherent (unambiguous) nrITS sequence may not be due to drying methods or processing problems. In cases of “unhomogenized” nrITS, the issue is inherent in the genome itself. Don’t drop the drying temperature too much…maybe around 105-115 degrees F.
We’ve learned from Dr. Karen Hughes about bacterial cloning which can successfully segregate some of the individual versions of nrITS from within a sample of mixed copies of differing nrITS. Unfortunately, it is not easy to find a lab/person that/who is both knowledgeable in the process and available and willing to take up an “after hours” job with amanitas.
This material has been received and accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing.
This has been dried & ready to pack for sending tonight.
At the moment, we are working our way through several species of sect. Vaginatae with regard to some basic sequencing. The results are very revealing as Naomi and I have mentioned on MO. We are finding that concepts for identification of temporary code numbers can include cryptic taxa, which is not surprising since decades of collecting have yielded many temporary codes, but have not deeply enriched the taxonomy of the section. The molecular methods are changing the understanding.
Because of we are continually discovering that our provisional concepts need revision, we are being as thorough as possible…trying to get sequences for all material in the Roosevelt herbarium that was thought (at any time in the past!) to belong within the scope of a temporary code or provisional name. I don’t see another way to do this as well as we can on a first pass through the herbarium (now approaching 6000 amanitas).
I hope folks can be patient with us. As you know we have on-going projects related to other sections of the genus in addition to the Vaginatae.
I found another one deeper in the woods, more brownish grey. Collected it also, but took no photographs of it.
Created: 2014-06-12 14:51:13 BST (+0100)
Last modified: 2014-06-13 02:03:06 BST (+0100)
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