Observation 170338: Amanita “sp-61” Tulloss, Kudzma & Wasilewski crypt. temp.

When: 2014-07-13

Collection location: Moon Lake Park, Pennsylvania, USA [Click for map]

Who: Dave W (Dave W)

Specimen available

Wyoming Valley Mushroom Club foray.

Proposed Names

92% (2)
Eye3 Eyes3
Based on chemical features: This species is the basis of the concept of sp-61" based on the data in this observation, the dried material in RET’s herbarium, and nrITS and nrLSU sequences derived from the aforementioned dried specimens.

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Eye3 = Observer’s choice
Eyes3 = Current consensus


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Thank you for the synopsis of finds.
By: R. E. Tulloss (ret)
2017-12-18 20:32:58 IST (+0530)

I look forward to hearing more about you 2017 collections. As you can probably see, Dr. Kudzma and I are trying to clear the queue of material that has previously been sampled and set to him. However, we can’t stop the sampling process for new material so we are beginning to create a new set of samples to move from the unsampled queue into Dr. Kudzma’s queue.

Very best,


I’ll sort through…
By: Dave W (Dave W)
2017-12-18 19:23:04 IST (+0530)

my 2017 collections of Vaginatae within the next few weeks. I know there were a few from Moon Lake; hopefully there’s more potential “sp-61” material. The area where “sp-62” was found produced virtually nothing in the way of Amanita this past year. Also, I’ll look through my older observations to see if anything may fit either of these concepts.

It was a good summer for rooseveltensis. I made multiple collections from a few different locations. But, maybe my most interesting grisette of last year was what looked like a very large rooseveltensis, but with spores significantly larger than rooseveltensis and with LARGER Q. Also found some rooseveltensis look-alikes (globose spores) growing nearby rooseveltensis.

That was my impression from your photos.
By: R. E. Tulloss (ret)
2017-12-16 10:40:58 IST (+0530)

Bright sun is a problem. I always try to photograph in open shade (with sky above me, and with the sun behind some convenient object.


Thank you…
By: I. G. Safonov (IGSafonov)
2017-12-16 10:32:53 IST (+0530)

…for weighing in, Rod. Yes, the cap of 281654 was of uniform brown, i.e., azonate. Unfortunately, I couldn’t take good pictures that reflect the shade of brown accurately (bright sunny days are the worst for picture taking), but if memory servers the caps were not red-brown.

My thought is that the two observation relate to different species.
By: R. E. Tulloss (ret)
2017-12-16 09:34:19 IST (+0530)

Amanitasp-61” can be distinctly red-brown and can be zonate a pallid zone on the pileus between darker red-brown regions over the center of the cap and on the ridges between the marginal striations.

That pattern is most suggestive of the colors and zones of the Eurasian species A. umbrinolutea.

Very best,


By: I. G. Safonov (IGSafonov)
2017-12-16 03:37:00 IST (+0530)

Do you think obs 281654 could also be “sp-61”? This collection is also in your hands.

Hello, David. This is another new species.
By: R. E. Tulloss (ret)
2017-12-16 01:12:23 IST (+0530)

No match fo either the proposed barcode gene or the nuclear ribosomal Large Subunit (nrLSU) gene when BLASTed against our local data base as well as GenBank. What do you think is the closest (mophologically) of the other zonate-capped amanitas in your collecting area?

I’ll put together a starter web page for this lady in the next week or so. She has the most common form of left hand terminal string for the nrLSU gene in Vaginatae.

This took a while; but we got good data thanks to Dr. Kudzma.

Very best,


Thanks Dave,
By: groundhog
2014-09-16 21:57:11 IST (+0530)

We have recieved this material and accessioned it to Rod’s Herbarium. We have scheduled it for DNA sequencing.

Created: 2014-07-16 07:00:45 IST (+0530)
Last modified: 2017-12-29 20:52:04 IST (+0530)
Viewed: 120 times, last viewed: 2018-02-13 21:21:20 IST (+0530)
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