Observation 171186: Agaricales sensu lato
When: 2014-07-25
No herbarium specimen

Notes: This fungi was found growing on and attached to a wet deadwood log in open light.

Images

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447957
Not sure about these three micrographs. I did find what I thought maybe spore but they were few in numbers and very small. The first image has been cropped heavily. Reagent was Lugals and Mag was X100 as was for all three.
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Proposed Names

28% (1)
Recognized by sight
78% (2)
Eye3 Eyes3
Recognized by sight
53% (3)
Recognized by sight
47% (2)
Recognized by sight

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Ian…
By: Richard and Danielle Kneal (bloodworm)
2014-08-24 19:27:29 CEST (+0200)

this also, may be of some help…

http://movehumanityforward.org/...

what danny said…
By: Richard and Danielle Kneal (bloodworm)
2014-08-24 18:05:45 CEST (+0200)

however, Ian stated it was mounted in Lugols…so it might just be a dextrinoid reaction…??
it does look a tad bit like a Bolete spore though…

Ian
By: Danny Newman (myxomop)
2014-08-24 14:07:05 CEST (+0200)

that spore has as good a chance of being a contaminant as belonging to the mushroom pictured, unfortunately. i’d recommend making a mount of a small, thin piece of gill, cut with the pileipellis on top and the gill edge below. if it’s dry, reconstitute the section by first applying ethanol, then, once evaporated, KOH. browse around the face and edges for cystidia, basidia, spores, etc., photograph them with enough neighboring tissue in frame to show how they differ from/contrast with surrounding cells. once you’ve done this, squash the mount (a pencil eraser works best, i find), and look again. you will see entire, “disembodied” versions of the same structures you first saw, if not new ones as well. if cystidia are present (not always an easy thing to determine), they are usually best measured at this step, since their entire length and width are visible, rather than being concealed in amongst the rest of the hymenium, pileipellis, or stipe.

Micrographs added

Not sure re the value to these as the spores? were extremely small and not available in numbers. All that I could find were single displays. Possible comments appreciated.

Created: 2014-07-26 01:35:49 CEST (+0200)
Last modified: 2014-08-23 10:50:43 CEST (+0200)
Viewed: 61 times, last viewed: 2015-09-23 21:07:36 CEST (+0200)
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