Observation 174286: Agaricales sensu lato

When: 2014-08-16

Collection location: Gainesville, Florida, USA [Click for map]

Who: Richard Kneal (bloodworm)

Specimen available


spores @ 1000x w/ KOH
spores @ 1000x w/ KOH
basidioles & cysitdia @ 400x w/ KOH
cysitdia @ 400x w/ KOH
cysitdia @ 400x w/ KOH
pileipellis a trichoderm @ 100x
pileipellis a trichoderm @ 400x
pleurocystidia koh @ 1000x
spores w/ Melzer’s 1000x
spores w/ Melzer’s 1000x
basidia, basidioles and pleurocystidia koh + Melzer’s 1000x

Proposed Names

92% (2)
Eye3 Eyes3
Recognized by sight
31% (2)
Recognized by sight
31% (2)
Recognized by sight
29% (1)
Recognized by sight

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus


Add Comment
By: Richard Kneal (bloodworm)
2014-08-26 11:12:02 -05 (-0500)

“Hi Richard,

I read through the link you sent re: Collybia aurea ()

I tracked down the long quote that you posted on that page on 2014-08-22 00:07:06 EDT; I found out that it comes from Jean Lodge et al. in the book Tropical Mycology, Volume 1, by Watling et al.

In that quote, Jean refers to a study published by myself, Vilgalys and several others in which rDNA sequences from a collection identified as C. aurea clustered with collections labeled Marasmius rhyssophyllus and Tricholomopsis rutilans (paper attached).

However, I am not familiar with this species: I’ve never collected nor examined it myself. The C. aurea data that were used in the attached paper and to which Lodge referred to in the Tropical Mycology book are from collection number RV.PR98/27. This labelling indicates that it was collected in Puerto Rico in 1998 by Rytas Vilgalys; I am guessing that he was in the field with Jean Lodge at that time.

I am copying this message to both Rytas and Jean. They may have more insights than I have to deal with your question/confusions.

All the best,

“Yes, we sequenced C. colorea from Puerto Rico, its a tropical Tricholomopsis. Callistosporium is something different….”

-Rytas Vilgalys

“Thank you for the ‘forward pass’.

Dear Richard Kneal,

I’ve looked at your photos, and there are a couple of things about the lamellae that don’t seem to match ‘Collybia’ aurea.
It may be the light coming through the lamellae, but they look orange, and C. aurea lamellae are white/cream.
Also, it looks as though the lamellae are decurrent, and the lamellae of C. aurea come almost straight in to the stipe.
Your fruit bodies also seem a bit big.

Take a look at this document that Susanne Sourell has put together with photos of her ‘C’. aura and ‘Marasmius’ rhyssophyllus from Brazil.
I will vouch for her IDs. She has posted these photos on Mushroom Observer.

Your fungus looks quite interesting. Were you able to collect and dry any of it? Or can you dry some of it when it fruits again?


D. Jean Lodge"

By: Richard Kneal (bloodworm)
2014-08-22 00:49:53 -05 (-0500)

i just emailed Dr. Moncalvo.
im sure he can shed some light on the situation.

also, maybe Bart Buyck might have some insight…

i am ready to defer to an agaricologist.
By: Danny Newman (myxomop)
2014-08-22 00:08:20 -05 (-0500)

but i don’t know who to ask offhand. I wonder if Vigalys or Moncalvo ever followed up with what was described in that paragraph you quoted…

in any case, the lot of them have been given sanctuary in a species list.

edit: J.M. Moncalvo and Rytas Vilgalys written.

By: Richard Kneal (bloodworm)
2014-08-22 00:00:54 -05 (-0500)

the last image clearly shows pleurocystidia on the gill faces…

my head hurts now.

the latter…
By: Richard Kneal (bloodworm)
2014-08-21 23:30:09 -05 (-0500)

was the case…

check out the last image.

basidia…same shape as the initial, “cheilocystidia.”

sorry for the screw up…

i saw that…
By: Richard Kneal (bloodworm)
2014-08-21 23:22:26 -05 (-0500)

on the name page…

but, i cannot find the original text.

it might be necessary to find the description for Collybia colorea.


the “cheilocystidia” i originally posted are basidioles and the “pleurocystidia” (they do look “hair-like”) are the cheilocystidia…

let me make another…more careful…mount…

By: Danny Newman (myxomop)
2014-08-21 23:16:59 -05 (-0500)

Pegler, D.N. “Studies on African Agaricales: I.” Kew Bulletin 21.3 (1968): 514-517 (see C. aurea name page)

and Kuo:


according to…
By: Richard Kneal (bloodworm)
2014-08-21 23:07:06 -05 (-0500)

what…or whom?

also…did you see this?

pleuro should be absent
By: Danny Newman (myxomop)
2014-08-21 22:50:41 -05 (-0500)

in both C. aurea and C. luteo-olivaceum. cheilo abundant in C. aurea, “inconspicuous” in C. luteo-olivaceum.

maybe this is neither…

last image…
By: Richard Kneal (bloodworm)
2014-08-21 22:37:12 -05 (-0500)

added…shows a basidia, basidioles and pleurocystidia…

at any rate…

i think the abundant, “cystidia” and pileipellis being a trichoderm rather than a cutis are very telling…

i will collect some, C. “luteo-olivaceum” and compare the micro and perhaps, DNA

By: Richard Kneal (bloodworm)
2014-08-21 22:19:34 -05 (-0500)

they may have turned “somewhat” purple on the walls…
but, nothing very distinct (they are really, really small).
i just looked at a slide that has been sitting in KOH for about 2 hours…
and…no change really.

i think what i posted is cheilocystidia…not basidioles.

i’ll post photos of basidioles in a Melzer’s mount in a minute…

agreed, richard
By: Danny Newman (myxomop)
2014-08-21 21:34:07 -05 (-0500)

two things to figure out:

are those basidioles or cystidia.

does the true C. aurea react to KOH on its cap surface

spore size and shape overlap for both Callistosporium and C. aurea, though the former’s should turn purple in KOH. did you observe any spore “purpling?”

By: Richard Kneal (bloodworm)
2014-08-21 19:59:46 -05 (-0500)


[edit]: also, added photos of spores in Melzer’s…no reaction.

just saw your edit…
By: Richard Kneal (bloodworm)
2014-08-21 19:45:39 -05 (-0500)

both pleuro and cheilo were abundant.
they were hard to differentiate them from the basidioles, as well…

i believe what i have posted are cheilo…
the pleuro seemed thinner and longer…more, “flexuous.”

By: Richard Kneal (bloodworm)
2014-08-21 19:11:02 -05 (-0500)

nice find.

thank you.

By: Richard Kneal (bloodworm)
2014-08-21 19:02:48 -05 (-0500)

let me see what i can find.

i also want to check the spores w/ Melzer’s.

By: Danny Newman (myxomop)
2014-08-21 18:57:02 -05 (-0500)

do you have a reference that describes C. aurea as having this KOH reaction?

edit: somehow reading this for the first time…:

The non-cellular structure of the pileus-surface indicates that this species is more correctly placed in the genus Collybia Kummer. Dennis (1951a) collected and described specimens from Trinidad which are identical with the African carpophores described above. He adopted the name Collybia colorea (Peck) Sacc., which is a later synonym of Callistosporium luteoolivaceum (Berk. & Curt.) Sacc. Although the two species are very similar in general habit and coloration, they differ markedly under the microscope as the spores and basidia of Callistosporium contain a distinctive purplish pigment, and the gill-edge is fertile. Collybia aurea may be recognized by the conspicuous and abundant cheilocystidia.

(emphasis mine)

That synonymy with Callistosporium is intriguing. I’m having trouble reconciling what it may mean for Collybia aurea.

My question for your then, Richard, is were these cystidia pleuro or cheilo?

By: Richard Kneal (bloodworm)
2014-08-21 18:40:03 -05 (-0500)


i added a photo of the KOH reaction.

By: Danny Newman (myxomop)
2014-08-21 18:24:53 -05 (-0500)

the more this Collybia aurea/Callistosporium/Marasmius rhyssophyllus/Collybia plectophylla business gets sorted out, the better.

I had snapped a photo of a color image and accompanying description of C. aurea I planned on posting to the name page, but can’t find it now. Bart Buyck wrote it up in some obscure French paper. There will surely be differences between and among these spp. we can pin down, observation by observation, starting with presence or absence of gill intervenosity and pileal surface KOH reaction (which I thought was negative for C. aurea…).

By: Richard Kneal (bloodworm)
2014-08-21 16:51:28 -05 (-0500)

no intervenosity…
KOH reacts purplish to black on the pileus.

i’m going to scope them tonight…

By: Danny Newman (myxomop)
2014-08-21 10:57:58 -05 (-0500)

any intervenosity? KOH +/- on the cap surface?

i support the C. aurea proposal in all but the flatness/collapsed look of the stipe and not having a clear look at the gills.

there are a handful of observations with these same stipes in common:

Observation 171069
Observation 54657
Observation 151571
Observation 54287
Observation 105422
Observation 54444

Created: 2014-08-16 16:32:33 -05 (-0500)
Last modified: 2014-10-14 18:35:35 -05 (-0500)
Viewed: 490 times, last viewed: 2018-03-14 00:56:54 -05 (-0500)
Show Log