Observation 174500: Amanita sect. Validae (Fr.) Singer
When: 2014-08-17

Notes: > A single specimen collected at an unknown location in FPP by J. or N. Burghardt.
> The mushroom is 8 cm tall, with the cap of 4.2 cm in diameter.
> Not sure if the yellow piece of volval material stuck to the stipe belongs to this mushroom.
> The annulus didn’t have the colors commonly seen in A. morrisii.

Microscopy:
Amyloid in Melzer’s reagent;
[20/1/1]: L x W = 8.1-9.8 (-10.7) x (5.1-) 5.3-6.7 μm;
L’ x W’ = 9.1 × 5.8 μm;
Q = (1.37-) 1.42-1.67 (-1.68); Q’ = 1.57; about 1/3 of the measure spores were “low range elongate” (Q = 1.60-1.68) and the rest were ellipsoid

In my experience, ellipsoid and broadly ellipsoid white spores are a pain in your know where to measure, especially if they are inamyloid, as focusing on a transparent and thin-walled object with the refraction ring interference becomes a growing annoyance. Furthermore, for some reason, very few spores of such a shape like to orientate themselves in the desirable lateral view with the apiculus visible (see pix). The next best alternative is a lateral view with the apiculus down, which can only be assessed once the true spore shape is recognized. This unfortunately eliminates the randomness of measurements. I frequently catch myself fishing for larger spores because their shape is less ambiguous. Smaller spores can tilt themselves and look more globose (while still appearing in focus on both ends) than they actually are, skewing your measurements.

Proposed Names

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Recognized by sight

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Eye3 = Observer’s choice
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Comments

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Thanks Igor,
By: groundhog
2014-10-14 11:23:51 CDT (-0500)

This material has been received and accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing.
-Naomi

Created: 2014-08-18 22:13:57 CDT (-0500)
Last modified: 2014-08-23 21:17:43 CDT (-0500)
Viewed: 52 times, last viewed: 2016-05-31 16:08:47 CDT (-0500)
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