Observation 175811: Lepiota subalba Kühner ex P.D. Orton
When: 2014-08-30
No herbarium specimen

Notes: Terrestrial in woodland area.

White spores.

Proposed Names

58% (1)
Eye3 Eyes3
Based on microscopic features: See comment.

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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I’ve got a lead…
By: Dave W (Dave W)
2014-08-31 23:57:33 PDT (-0700)

on obtaining some Meltzer’s. So before messing around with other solutions, I’m gonna check this source.

Thanks, Dave
By: I. G. Safonov (IGSafonov)
2014-08-31 23:32:19 PDT (-0700)

Before I was finally able to get my hands on some Melzer’s I had good results with Lugol’s. The trick is to use a fairly dilute reagent (<2.5%) and allow excess iodine evaporate or get destroyed photochemically and then reconstitute spores in water. Yes, I understand that Lugol’s doesn’t have a clearing agent in it, but if the goal is to improve visualization of spore surfaces at high magnification, it does the job pretty well. I posted many russulas this summer that showed classic amyloid reaction (grayish blue) to IKI. Lugol’s also produced a perfect dextrinoid reaction for me in Leucoagaricus americanus. Yes, it’s more messy and time consuming to prep slides with Lugol’s, but it’s the next best alternative to Melzer’s. I remember reading some paper claiming that Lugol’s cannot be used as the reagent of choice for reliable amyloidity tests, but it could just be a methodology problem. I will be willing to test a range of Amanita subgenus Lepidella with my modified protocol next year and report on my finding in case of a success.

Bullet-shaped spores…
By: Dave W (Dave W)
2014-08-31 22:58:38 PDT (-0700)

suggest L. subalba. But according to what I have recently learned, I would need to see that the spores are spurred in order to achieve a high level of confidence. I think if I had some Meltzer’s maybe I could see this, as the dextrinoid reaction would possibly help highlight this detail.

Subalba seems promising to me. Other possibilities that have been suggested have differently shaped spores.

Cylindric spores
By: I. G. Safonov (IGSafonov)
2014-08-31 22:22:16 PDT (-0700)

Any chance you can use microscopy to arrive at a species name, Dave?

Created: 2014-08-31 16:46:08 PDT (-0700)
Last modified: 2014-08-31 22:59:31 PDT (-0700)
Viewed: 33 times, last viewed: 2016-07-11 02:59:08 PDT (-0700)
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