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I’m not surprised that you are getting something that is not an Amanita from your BLASTing of your derived sequence. There are probably lots of yeasts, bacteria, a variety of “hyphomycetes” chowing down on the specimen considering its condition. If the match has a very good grade (high percentage of overlap with your sequence) and the percentage of the match is very high, you might really have the Candida present; and, in that sense, the BLAST produced some information that is “correct” or close to “correct.” You didn’t get a sequence from the organism that your eyes can detect, but you did detect an organism which either is present or has a close-ish relative present.
I’m sorry, it would have been cool to get something from the Amanita.
Thats interesting because I extracted DNA from the gill and the top BLAST match was Candida tritomae which is obviously incorrect! Someone just mentioned to me the ovoid spores appear to be from a Russula so at least that part is sorted out.
The cylindric spore depicted in your images (length/width ratio of about 2.45) is probably the Amanita’s spore, possibly even a mature spore that remained on the fruiting body but was produced before the setting in of the advancing state of decay. The other spores are too small for normal North American amanita spores in a normal mature specimen. They could be spores of something growing on the decaying fungus or they could be spores of the fungus in an advanced state of senility. An Amanita spore with length 8.3 micrometers or smaller has a very high probability of being from a tropical rain forest species. This is my reason for thinking the smaller spores do not represent normal state spores from your specimen.
Created: 2014-11-27 16:37:18 CST (-0500)
Last modified: 2014-12-28 09:58:28 CST (-0500)
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