Observation 190682: Amanita castellae Tulloss and Kudzma nom. prov.


Douglas fir is dominant in this area. About 2200 ft.

Proposed Names

91% (2)
Based on chemical features: Sequences will be posted here. The species is distinctive from all species that have been sequenced. The concept of the new provisional taxon will be based on the voucher material from this observation.

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Eye3 = Observer’s choice
Eyes3 = Current consensus


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You’re very welcome.
By: R. E. Tulloss (ret)
2018-03-03 06:31:06 CST (+0800)

It’s my pleasure to be sent more cool examples of the Vaginatae from all over the world.


That’s very exciting, Rod.
By: (Aaron Cena) (mountainplayer)
2018-03-02 00:32:40 CST (+0800)

We will do as suggested and compile a list of trees near to the collection.

I’ll post the list here.

Had a snow event last night and this morning, with up to 30" expected to accumulate by Saturday. Will delay the project a bit.

Thanks for your work on this, and for letting us play a small part in it.

I suggest making a list of the trees within a circle having a radius of…
By: R. E. Tulloss (ret)
2018-03-01 10:23:41 CST (+0800)

20 -30 paces or so and centered on the mushroom(s) to be collected.

I decided to make this specimen the basis of a new species concept because we have an image, dried, material and DNA of you material.

Very best,


These photos were taken at the collection site.
By: (Aaron Cena) (mountainplayer)
2017-08-10 06:34:48 CST (+0800)

This location is dominated by Douglas fir. There are some scattered big leaf maple, an incense cedar every so often, and a Ponderosa pine (or some other three needle pine) here and there.

Thanks to you, I realize this might be something special. I’ll really scrutinize the nearby trees when I encounter this mushroom this fall. How far away would a tree be before it was unlikely as a mychorrizal partner?

We have located a herbarium specimen with an nrLSU sequence very similar to…
By: R. E. Tulloss (ret)
2017-08-10 06:04:45 CST (+0800)

the one we have from this material. We are looking for a photograph. The collector’s notes suggest that there were color bands on the cap. Also, it is noted that it was growing with Doug Fir at a relatively high elevation in Arizona.

Very best,


The photographs include pine needles.
By: R. E. Tulloss (ret)
2017-08-10 05:49:24 CST (+0800)

Did you photograph the material at a site other than the collecting site?

Very best,


When you see this critter again, please measure the stem…
By: R. E. Tulloss (ret)
2017-03-31 06:30:46 CST (+0800)

and the portion of the stem that is underground. It looks as though the the volval sac is entirely situated below the surface of the soil. I’d like to know more about the mushroom’s dimensions.

Very best,


Aaron, thanks for you response and your offer of more material.
By: R. E. Tulloss (ret)
2017-03-31 06:27:29 CST (+0800)

It seems that 187726 has not been put into the sequencing queue. We should certainly do that. I hope to do more sampling for the queue. In the next several months. I’m building up a to do list.

Very best,


Thanks, Rod. Glad that this turned out to be something different! I sent you
By: (Aaron Cena) (mountainplayer)
2017-03-31 01:32:36 CST (+0800)

another sample of what I believe is the same species: http://mushroomobserver.org/187726?q=AaE

Note that on my MO Obs notes for 187726, I reference yet another observation. I don’t know if I collected/retained a sample of that material.

These tend to show up every year in the same small area. If they show up again this fall, I’ll take special care with photos and collect several specimens for you.

Hello, Aaron.
By: R. E. Tulloss (ret)
2017-03-31 00:31:22 CST (+0800)

You found a real gem as far as I’m concerned.

We attempted to derive two gene sequences from your material and have just gotten the results. For the “proposed fungal barcode” gene we got partial results. Very good ends with a large gap of poor quality data in the middle.

This often indicates that there are multiple areas of variation in the copies of the gene that exist in your mushroom. The proposed barcode gene (aka “nuclear ribosomal internal transcribed spacer” or “nrITS”) appears in DNA in many copies called the “ribosomal DNA repeat.” Often these copies are all identical (this is when the “barcode” works like a barcode ought to work).

In Amanita (and other genera) the separate copies are often not identical. In the case of the present material, we got a very nice “right hand end” (3’ terminus) of the barcode with a goodly chunk of the neighboring gene attached. The neighboring gene is called the “nuclear ribosomal large subunit” ( or “nrLSU” aka “28S”). By combining the data from four different sequencing “reads,” we were able to get over 1400 characters of nrLSU beginning at what we take to be the first character.

From this data we can see that your material is in the grouping that I’ve called “the penetratrix group” here on MO and other places. This is a group within section Vaginatae that includes a variety of rather unusual macroscopic forms including deeply penetrating stems and (even) partial veils (one case so far in North America). See:


California material in this group has only come my way once before…at a SOMA foray many years ago. The specimen in question was of very large size, with a very dark brown cap, and a volva suggesting A. constricta. That is to say, that material was rather different from yours in gross appearance. Hence, I think it safe to say that you have found another species of penetratrix group from California. Since, I’m studying the Vaginatae at present, your collection is of particular interest to me.

Thank you very much for generously contributing the material.

Very best,

Rod Tulloss

Thank you, Aaron.
By: R. E. Tulloss (ret)
2014-12-13 06:42:45 CST (+0800)

The dried collection was received here in Roosevelt.

Thank you for your interest in our work.

Very best,


I would be very interested in examing your material if a portion of it is available…
By: R. E. Tulloss (ret)
2014-12-01 10:45:36 CST (+0800)

for research.

Very best,


Created: 2014-12-01 07:47:10 CST (+0800)
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