Observation 193058: Amanita crassiputamen Tulloss nom. prov.
When: 2014-12-08

Notes: Growing on the ground with Live oak being the most dominant tree.
Total height about 10 cm.
Spore print white and spores not amyloid.
Spores ~ 9.0-11.0 X 8.1-10.0 microns, globose to subglobose and smooth.
Q(ave) = 1.07.
Only a single fruit-body but thought the light color and thick volval patch were interesting and rather unique for the Grisettes in this area.

Proposed Names

76% (2)
Eye3 Eyes3
Recognized by sight
Used references: Comments by RET

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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That’s a good way of making a judgment call.
By: R. E. Tulloss (ret)
2017-06-16 18:23:43 PDT (-0700)

Good idea, Ron.

Very best,

R

Thanks Rod,
By: Ron Pastorino (Ronpast)
2017-06-16 15:16:50 PDT (-0700)

I’ll go with A. crassiputamen as “Promising” for now. Many “Promising” id’s on MO with a lot less back up information.

Sorry that we didn’t report the date of accessioning….EDITED
By: R. E. Tulloss (ret)
2017-06-15 09:39:26 PDT (-0700)

We don’t record that date on herbarium labels; therefore, I can’t correct the record on the observation.

From the container in the herbarium, I can tell you with high probability that the collection was accessioned in late December 2014 or early January 2015.

Very best,

Rod

This material was accessioned & we have both nrITS & nrLSU sequences…EDITED
By: R. E. Tulloss (ret)
2017-06-15 09:30:29 PDT (-0700)

…from it.

The nrITS is very similar to the sequence we have from the1989 collection of A. crassiputamen.

By “very similar” I mean the pairwise identity is 100%. There are two positions in the sequence where one of the pair is ambiguous (say, C-or-T) and the other is not (say, C). This is considered pairwise identity since in a case of heterogeny a nucleotide position may be read (same example) as C or as T or as C-or-T.

Unfortunately we have no nrLSU from the older specimen. We will look into getting one with Linas’ current techniques.

Since we have a full techincal description of that material, I will check at least a few of the key anatomical characters against your material. The cap of your material is paler that the grayish brown cap reported for RET 092-1 (1989 collection). This could be due to natural variation…but, of course, we only have two examples in hand.

If I have any progress on this matter, I will advise you further.

Very best,

Rod

Your material has arrived in Roosevelt.
By: R. E. Tulloss (ret)
2014-12-15 14:05:46 PST (-0800)

Thank you, Ron.

The material will be accessioned in the near future.

Very best,

Rod

That’s great, Ron.
By: R. E. Tulloss (ret)
2014-12-11 04:26:03 PST (-0800)

I look forward to seeing the material.

Very best,

Rod

Sure Rod,
By: Ron Pastorino (Ronpast)
2014-12-10 19:15:20 PST (-0800)

I’ll sent the whole thing(two-halves).

This looks very interesting.
By: R. E. Tulloss (ret)
2014-12-10 18:52:23 PST (-0800)

Would you be will to share a bit of this one?

Very best,

Rod

Created: 2014-12-10 17:32:50 PST (-0800)
Last modified: 2017-06-16 15:18:51 PDT (-0700)
Viewed: 87 times, last viewed: 2017-06-20 17:28:57 PDT (-0700)
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