Observation 194406: Polyporales sensu lato
When: 2014-12-19

Notes: Similar fungi loaded to MO Obs: 45358, but I looked at similar images an am not confident that this is the same. (Hydnellum). The fungi was well attached to either a dead or living root on a bank area off the forest road. The fungi was firm without odour and I managed to obtain a spore print that was “Off White tending towards Light Brown”. I did several micrographs without much success. The fungi appears to be Ascomycete by the results in the micrographs. It was a single specimen on the verge of the Eucalyptus forest. The fungi was in direct open sunlight position, but in good condition..e

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By: Richard and Danielle Kneal (bloodworm)
2014-12-23 16:55:07 CET (+0100)

Ian got that idea from me. see: http://mushroomobserver.org/192181
I have used H2O2 in the past, before I had KOH.
it actually doesn’t work that bad, although I have no idea what changes it might make to the cells in the long run.
you do need to rinse the specimen with H20 after a drop of H2O2 is applied, though…
here is an example of a Gymnopilus section hydrated in 3% H2O2 and then rinsed and mounted in H20.

hydrogen peroxide?
By: Danny Newman (myxomop)
2014-12-23 08:48:21 CET (+0100)

good for cuts and nicks from sectioning fungi, but no good under the microscope. you want potassium hydroxide.


Thanks for your patience an such helpful advice. I went straight to max mag thinking it would be better for viewing. I will take onboard what you have said as it makes good sense. (I picked up some Hydrogen Peroxide today and have printed out the last notes you provided. I will see how I go as I think this specimen is worth the effort.)

By: Danny Newman (myxomop)
2014-12-23 07:59:04 CET (+0100)

no poroid fungus such as this one is an ascomycete. it is often helpful to know what to look for and what one is looking at when performing mushroom microscopy, so as to not be overwhelmed by a mass of indecipherable tissue and structures. you were seeing hyphae belonging to this polypore, not asci, of which there were likely two or more types. telling them apart can be difficult, as can simply making good mounts of polypores in general, as the tissue is typically quite tough and unsquashable. some polyporologists will leave a piece of tissue in KOH for up to several days in order to sufficiently soften it for mounting.

I echo my earlier recommendation that you lead us into your micrographs with photos of smaller to larger magnifications. this will allow us to point out interesting structures/areas you may not have previously seen, as well as determine the quality of the mount. otherwise, it may be difficult to instruct you through comments alone.

Created: 2014-12-23 01:17:56 CET (+0100)
Last modified: 2015-03-11 10:30:19 CET (+0100)
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