Observation 198098: Trichoglossum hirsutum (Pers.) Boud.
When: 2015-02-03
No herbarium specimen

Notes: Found in mixed woods by Patrick Hamilton.
Spore print blackish.
Spores ~ 113-127 X 5.0-6.0 microns with 15 septate.


Spores in KOH @ 1000X.
Spore in KOH @ 1000X.
Asci and spores in KOH @ 400X.
Setae and asci in Congo red @ 400X.
Asci in Congo red @ 1000X.

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Eye3 = Observer’s choice
Eyes3 = Current consensus


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It’s an old used one I bought
By: Ron Pastorino (Ronpast)
2015-02-08 00:29:21 JST (+0900)

from UC Davis, a Leitz HM-LUX.

Incredibly good sharp photos!
By: Martin Livezey (MLivezey)
2015-02-08 00:05:22 JST (+0900)

What microscope are you using? Maybe you have told me before…,

By: zaca
2015-02-06 09:37:35 JST (+0900)

it seems a good technique, that soon I will try.
Thanks, Ron, for the explanations.

I just placed the heads of the fruit-bodies
By: Ron Pastorino (Ronpast)
2015-02-06 09:32:00 JST (+0900)

on a clean surface under glass overnight. There were enough loose spores to give a decent print which I used to make the first two spore photos.

I don’t understand,
By: zaca
2015-02-06 09:05:23 JST (+0900)

what do you mean by spore print in this type of fungi?

I agree Zaca, but I don’t know how much
By: Ron Pastorino (Ronpast)
2015-02-06 08:53:02 JST (+0900)

weight one can put on immature spores still in the asci.
All of the spores I checked were from the spore print and they were consistently with 15 septate.
Also just added a couple more micro photos.

OK, Thanks, it was just a warning,
By: zaca
2015-02-05 19:56:34 JST (+0900)

because in the last photo there is a spore (on the middle right) which has clearly 12-septa and some others within the asci cannot have 15-septa as well.

According to the new “Ascomycete Fungi of North America”
By: Ron Pastorino (Ronpast)
2015-02-05 10:25:56 JST (+0900)

by Beug and the Bessettes, Trichoglossum variabile has mostly 9-14 septate spores.
Initially, I was getting 16 on all my spores but then realized everybody must be counting just the vertical lines and not the individual segments.

Did you considered …
By: zaca
2015-02-05 10:03:39 JST (+0900)

Trichoglossum variabile?
Trichoglossum hirsutum has consistently 15-septate spores, which is not the case of some in the attached photos.

Created: 2015-02-05 09:56:44 JST (+0900)
Last modified: 2015-02-06 11:49:17 JST (+0900)
Viewed: 175 times, last viewed: 2016-10-27 22:49:00 JST (+0900)
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