Observation 199931: Laricifomes officinalis (Batsch) Kotl. & Pouzar
When: 2015-03-02

Notes: Two(still growing) Agarikon on old growth Western Larch substrate.

2nd conk on right side of log was frozen in ice- but leading edge was still soft, cheesy and tasted bitter as hell.

Largest conk(in first set of images) was sampled from and is in culture.

Elevation: Approximately 3000ft.

Temp: low 30’s.

Additional images of home Microscopy(analyzed on 3/2/15) added below

Basidia, sterigmata, Round spore shape (not ellipsoid as with F.officinalis), Hyaline, thick walled cells. Could this actually be a F. pinicola? spore description for F.officinalis in all keys claim ellipsoid(lemon almost) spore shape and not round.

Microscopic results imply Fomitopsis pinicola-see key below.

All other indicators, including taste(extremely bitter), context(old growth larix occidentalis) and friability are correct for L. officinalis.

Master Key oF Polypores From PNW key council:
http://www.svims.ca/council/Polypo.htm

Note: Specimen is available for further study* substrate is located and marked within 1hr. of home base.

UPDATED 3/8/2015: Laricifomes officinalis confirmed by anonymous aid. Spores lemon shaped(I just have a low quality digital scope). Apologies for the confusion in earlier posts.

UPDATED: 3/8/2015: MULTIPLE IMAGES ADDED BELOW OF PDA SLANT INOCULATIONS .

UPDATED: 3/24/15: PDA AGAR slants with newly establishing L.officinalis mycelium.(very slow growing, as we knew going in).

UPDATED: 7/8/15- Slants with fluffy, white mycelium after overrunning Penicillium.

UPDATED 11/6/2015- FLUFFY, RADIANT MYCELIUM HAS THRIVED BELOW SMALL STERILE inoculant OF L.officinalis finally over ran the initial penicillin contaminant. Substrate almost completely filled with healthy mycelium/hyphae inside slant.

Species Lists

Images

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Left side fruiting body on old growth larch substrate.
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Left side fruiting body on old growth larch substrate.
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Left side fruiting body on old growth larch substrate.
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510020
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(2nd)Right side fruiting body found at the opposite end of the old growth substrate.Completely frozen, fresh growth of pore layer still visible underneath. Interesting how both fruiting bodies were angled and growing from right to left – Possibly a wind influence, heat influence, or both.
510021
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(2nd)Right side fruiting body found at the opposite end of the old growth substrate.Completely frozen, fresh growth of pore layer still visible underneath. Interesting how both fruiting bodies were angled and growing from right to left – Possibly a wind influence, heat influence, or both.
510022
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(2nd)Right side fruiting body found at the opposite end of the old growth substrate.Completely frozen, fresh growth of pore layer still visible underneath. Interesting how both fruiting bodies were angled and growing from right to left – Possibly a wind influence, heat influence, or both.
510505
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Spores lemon shaped, to slight ellipsoid, thick walled cells(of hyphae).
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510507
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510508
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510509
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510515
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511171
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UPDATED 3/7/15 Sterile media(PDA)-ready for inoculation.Used pseudo field methods for sterilization using milar clear plastic sheets as hoods.Arm, gloved protection, lots of 99%alcohol, lysol and bunson burner for instruments, mouth cover, and repeated disposal and sterilization with each cut or to...
511172
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UPDATED 3/7/15 Sterile media(PDA)-ready for inoculation.Used pseudo field methods for sterilization using milar clear plastic sheets as hoods.Arm, gloved protection, lots of 99%alcohol, lysol and bunson burner for instruments, mouth cover, and repeated disposal and sterilization with each cut or to...
511174
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Two slants on left side were inoculated w/conk above near bumping lake(199931herbarium1)3 slants on right side were inoculated from conk in this below observation: http://mushroomobserver.org/200282?q=2WgZL
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3/24/15 UPDATED: beginning to establish white hyphae clouds. Over running black mold and lax septic transfers.
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3/24/15 UPDATED: beginning to establish white hyphae clouds. Over running black mold and lax septic transfers.
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UPDATED GROWTH 7/8/15. Fluffy, healthy, all white mycelium in slants outcompeted and overran Penicilium in culture.
536271
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UPDATED GROWTH 7/8/15. Fluffy, healthy, all white mycelium in slants outcompeted and overran Penicilium in culture.
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©DREW HENDERSON 2015. UPDATED ON 11/6/2015.
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©DREW HENDERSON 2015. UPDATED ON 11/6/2015.
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©DREW HENDERSON 2015. UPDATED ON 11/6/2015.

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Created: 2015-02-27 15:49:07 PST (-0800)
Last modified: 2015-11-06 15:00:00 PST (-0800)
Viewed: 161 times, last viewed: 2016-09-19 14:51:27 PDT (-0700)
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