Observation 200282: Laricifomes officinalis (Batsch) Kotl. & Pouzar
When: 2015-03-06

Notes: Old sporophore attached to what I perceived to be fresh growing tissue. When I went to extract the old specimen, the entire shebang came with it… I decided to consult a good friend of mine who specializes in lab sanitizing and methods for cloning in PDA media.

This fresh tissue sample was absolutely covered in what we think to be possibly Aspergillus niger on most of the undersurface)pore structure). I see this commonly on most all Agarikon that are old and fallen from substrate. They always exhibit the “grey pockets” more towards the inner center of the conk. This phenomenon has probably not been noticed or mentioned in guidebooks because this species is usually only encountered once or twice in someones lifetime. Luckily, I have had experience in the field with more that 30 locations in WA state alone and continue to seek out new locations week to week. I saved the A. niger in a bag and sliced most all of the mold from the pore surface. For those interested in studying extremely potent black mold that can withstand Laricifomes officinalis’ powerful antibiotics/antivirals- give me a call or email.

Today- 3/8/2015- I inoculated 3 slants we prepared yesterday with sterile tissue samples- Aseptic transfer.

Elevation of extraction: approx. 5000ft. Host tree: Pseudotsuga menziesii(Douglas fir).

Temp: low 50’s.

3/24/15 UPDATED SLANT IMAGES OF INOCULATED PDA BELOW:

UPDATED 7/8/15: “Sweating” sample after outcompeting black mold and Penicillin.

Species Lists

Images

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35 feet from forest floor(long side felled fir was my ladder)
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Further view from a cross canopy snag.
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Close up of both friable sporophores(Laricifomes officinalis) after accidental removal from Pseudotsuga menziesii. Small broken conk on left-donated to SCMS herbarium, WA.
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Close up of both friable sporophores(Laricifomes officinalis) after accidental removal from Pseudotsuga menziesii. Microscopy of ellipsoid spores and hyaline hyphal cell structure.
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Close up of both friable sporophores(Laricifomes officinalis) after accidental removal from Pseudotsuga menziesii. Microscopy of ellipsoid spores and hyaline hyphal cell structure.
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Close up of both friable sporophores(Laricifomes officinalis) after accidental removal from Pseudotsuga menziesii. Microscopy of ellipsoid spores and hyaline hyphal cell structure.
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Microscopy- ellipsoid spores. Transient Aspergillus visible.
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Microscopy- ellipsoid spores.
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Microscopy- ellipsoid spores.
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3 slants on right side are inoculated with material from the above observation.2 slants on left are from observation linked below:http://mushroomobserver.org/199931?q=2WgZL
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Possible strain of A.niger on bottom of growing pore surface. Cut pieces are bagged, labeled and available for further study.
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Possible strain of A.niger on bottom of growing pore surface. Cut pieces are bagged, labeled and available for further study.
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Possible strain of A.niger on bottom of growing pore surface. Cut pieces are bagged, labeled and available for further study.
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3/24/15UPDATED GROWTH: Most of these fresh tissue samples were inoculated into AGAR with black mold to see overrunning possibilities. Inoculated ASEPTIC T smaples are thriving. We will see about the Black mold(septic) transfer success.
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3/24/15UPDATED GROWTH: Most of these fresh tissue samples were inoculated into AGAR with black mold to see overrunning possibilities. Inoculated ASEPTIC T smaples are thriving. We will see about the Black mold(septic) transfer success.
513255
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3/24/15UPDATED GROWTH: Most of these fresh tissue samples were inoculated into AGAR with black mold to see overrunning possibilities. Inoculated ASEPTIC T smaples are thriving. We will see about the Black mold(septic) transfer success.
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UPDATED 7/8/15.
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UPDATED 7/8/15.

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Created: 2015-03-08 09:45:13 PDT (-0700)
Last modified: 2015-07-08 15:02:06 PDT (-0700)
Viewed: 232 times, last viewed: 2016-11-17 19:25:25 PST (-0800)
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