Observation 203284: Pestalotiopsis microspora (Speg.) G.C. Zhao & N. Li
When: 2015-04-24
Who: MSchink
No herbarium specimen

Notes: Unknown fungi cultured from the skin of Ganoderma meredithae from observation 183909

Growth on MEA is light,fluffy and cottony, slightly dendritic. Beige at first gaining a yellow tinge in age. Black liquid pools grow after a few weeks. These pools turned out to be spore masses.

Spores are oblong, appear slightly jointed tapering to a point on one end and two flagella looking extensions on the other.
Spore sizes as follow.

14.92x 5.36
19.15x 5.43
13.24x 4.95
15.14x 5.20
16.04x 4.93
15.67x 5.06
17.17x 5.19
19.10x 4.37
15.89x 4.41
13.71x 4.29
17.11x 5.57

Unsure of what this is.

I will see if I can find some older photos from when it was young.

Proposed Names

30% (2)
Recognized by sight
60% (2)
Eye3
Based on microscopic features: 5 celled conidia, 3-4 median cells darkened and concolorous, 2-3 flagella like appendages on one end, a single flagella like appendage on the other.
55% (1)
Recognized by sight

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Please do not destroy your proposed names to remove other people’s votes.
By: Byrain
2015-04-26 08:50:37 PDT (-0700)

Alan.

Please send it to me for sequencing
By: Alan Rockefeller (Alan Rockefeller)
2015-04-26 04:20:49 PDT (-0700)

I will also test it for its ability or lack thereof to degrade plastic.

Neopestalotoiopsis
By: MSchink
2015-04-25 18:01:21 PDT (-0700)

is the easy one, differentiated because Neopestaliopsis has versicolored median cells, with one or more cells being darker in color than the others.

I concede that Pestalotiopsis and Pseudopestalotiopsis is apparently nearly indistinguishable via morphology, but the paper does make note that the colored median cells are typically darker in Pseudopestalotiopsis. In this observation though the colored cells seem to be a lighter color. Though I do not have anything else to reference them to.

I am willing to give a sample to someone willing to sequence it. I am not sure if the culture on the plate is still living though as it has been sitting on a shelf for a few months before I got around to scoping it.

Nice paper.
By: Byrain
2015-04-25 16:52:47 PDT (-0700)

Its a lot to digest, how are you ruling out Neopestalotiopsis & Pseudopestalotiopsis? Best I can tell from a initial skim is that they require DNA to distinguish from Pestalotiopsis. Amphisphaeriaceae at least seems like a safe name.

Ok,
By: MSchink
2015-04-25 10:44:15 PDT (-0700)

Here is why I did not Challenge Alan’s proposal, although identifying it down to species probably is not feasible given some of the papers I looked at showing a broad diversity in Pestalotiopsis.

These fit every diagnostic criteria for Pestalotiopsis that I have come across without access to that book.

5 cells, with 3 or 4 median cells darkened. 2-3 extensions on one end and one from the other. Septums do not appear to be distoseptate ruling out Pestalotia, Pestalotia is also mentioned as having 6 segments. It also differs from Truncatella in having 5 cells instead of 4. It is distinct from Neopestalotiopsis by having concolorus median cells.It is distinct from Monochaetia by having 2 or more appendages on the one end.

Every thing I find online seems to confirm this concept. Unless I am missing something I cant find any reason for this not to be Pestalotiopsis. Alans proposal might be incorrect but I think hes on the right path.

http://species-id.net/wiki/Pestalotiopsis

http://www.sciencedirect.com/...

Not likely a good name to use….
By: Byrain
2015-04-25 07:51:11 PDT (-0700)

There are lot of things with conidia like this, but then you probably don’t care and just want a name on it or I wouldn’t have to explain this twice…

The answer could easily be in this 1100 page book, if anyone has access.

http://www.mycolog.com/coelorev.html

https://www.worldcat.org/...

Added photos of rehydrated spores.
By: MSchink
2015-04-24 21:09:21 PDT (-0700)

They seem to match Alan’s.

Created: 2015-04-24 02:21:15 PDT (-0700)
Last modified: 2015-04-26 11:15:48 PDT (-0700)
Viewed: 260 times, last viewed: 2016-11-10 09:09:32 PST (-0800)
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