Observation 204192: Cortinarius (Pers.) Gray
When: 2015-05-09

Notes: Small single cluster of fungi growing on moss covered bank on Eucalyptus forest. Youngest specimen displayed dark brown caps, and as maturity increased colour becomes lighter.Stipes white and thick and loosely rooted in the soil. Gills brown and SD.

Images

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Both Micrographs ..Reagent Melzers x 100. 2ND image contrast increased.
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Both Micrographs ..Reagent Melzers x 100. 2ND image contrast increased.
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10X,40X and 100X in water solution. Images cropped and edited in PS. (just & especially for my good friend DV. huh.) kk
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10X,40X and 100X in water solution. Images cropped and edited in PS. (just & especially for my good friend DV. huh.) kk
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10X,40X and 100X in water solution. Images cropped and edited in PS. (just & especially for my good friend DV. huh.) kk
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Two images using Oil (X100 objective)
Images edited (processed ) slightly differently. Show minor differences.
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Two images using Oil (X100 objective)
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Proposed Names

-14% (2)
Recognized by sight
31% (2)
Recognized by sight
53% (3)
Eye3 Eyes3
Recognized by sight: Not seeing any spore deposits or evidence of cortinas on upper stipes. But lower stipes appear to show deposits which may have originated as partial veils. Not all Corts show evidence of PV deposits.

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
The oil immersion photo looks nice, Ian.
By: Dave W (Dave W)
2015-05-12 23:29:15 CDT (-0500)

I believe the round central areas are oil drops.

Microscopy is not my strength. For my micro-photos I just point my camera into the eyepiece on the scope and snap a bunch of photos. Sometimes I get lucky and a few of the photos show some details. So please don’t think I’m being critical of your photos.

I agree with Debbie about holding off on the Meltzer’s mounts until after you get a good look at spores in a clear liquid. I usually use 4-5% KOH solution. I have been told this supports inflation of (possibly dry) spores to natural dimensions. But for some types (like Gyromitra species) spore ornamentation may come through better in a water mount. Meltzer’s really helps with the light-spore types that react.. Amanitas subspecies Lepidella, Russulas, Lepiotas and allied genera. The Metltzer’s color reaction sometimes highlights spore ornamentation… like with Russula or Lactarius.

I use Congo red stain to look for cystidia.

As per the spore info, I’m leaning away from my Cort proposal. Let’s see if anyone with better knowledge of Cort spore morphology weighs in on this one.

Oil Immersion micrographs added
Ian, the magnification looks okay.
By: Dave W (Dave W)
2015-05-12 21:02:13 CDT (-0500)

I think your camera may be having some difficulty focusing on the micro.

Smooth spores with oil drops (possibility for the central circles) seems to point away from Cortinarius. (But this is a large genus with fairly diverse spore morphology.)

Spores

From what I see they are ovate,(oval), smooth & with a central circular pattern in each pore. All pores appear to be similar with some showing more pattern than others. When I increased the contrast with Melzer’s, there seems to be some darker spots near the edges. This didnt appear with water solution. Dave, I am not sure how to improve the magnifcation although I have tried several positions of contast with the diaphram slider, and adjusting the light through the slide.
I could try oil immersion if you think that may improve the spore images.

I’m wondering about the surfaces of the spores.
By: Dave W (Dave W)
2015-05-12 20:04:35 CDT (-0500)

Ian, would you call the spores smooth? Or if otherwise, how would you describe the spores? Roughened? Warty? I can’t tell from the photos.

good to see that you have Melzer’s at hand, Ian!
By: Debbie Viess (amanitarita)
2015-05-12 11:09:35 CDT (-0500)

but frankly, we could use a plain water mount here, to see the original spore color. At least this is producing spores! ;)

You might also want to try a smash mount so as to get those spores free-floating. Grab a gill portion, drop into a small drop of water on your slide, apply cover slip, then crush a bit with a pencil eraser. You want that gill to spread out into the water. Then search for those free floating spores, without that gill trama backdrop.

Thanks for your follow-up! You da Man in OZ!

Micrographs added

May help. kk

even mature gills …
By: Debbie Viess (amanitarita)
2015-05-11 20:25:36 CDT (-0500)

show no rusty brown color.
no rusty brown spore drop anywhere on any stipe.

no sign of a cortina, on any of many.

weird island mycoflora, who knows what it is?

but if I had to pick a random looks kinda like … I’d say Cortinarius, too.

Debbie, just curious…
By: Dave W (Dave W)
2015-05-11 18:31:04 CDT (-0500)

What leads you away from Cortinarius here?

spore drop color would help.
By: Debbie Viess (amanitarita)
2015-05-11 18:07:57 CDT (-0500)

I don’t think that this is a cort.

Created: 2015-05-11 02:18:20 CDT (-0500)
Last modified: 2015-05-13 02:39:06 CDT (-0500)
Viewed: 145 times, last viewed: 2016-03-05 23:30:44 CST (-0600)
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