Observation 204417: Neolentinus Redhead & Ginns
When: 2015-05-15

Notes:
OMS 1000043
Collector(s): Nathan Parr
Determined: 2015-May-16
Habitat: Conifer
Aspect: none
Growth Habit: Single
Substrate: Soil
Closest plant:
Other Habitat & Locality Notes: closest tree: conifer
Odor: Nondescript
Taste: Nondescript
Spore print:
Other Specimen Notes:
Gills: edges serrated; anastomose close to stem; appear to again anastomose near cap edge, so 2-3 rows of gills
Chemical: KOH yellowish on gills (see photo); KOH negative on stipe; FeSO4 negative on cap and stem
Spores (preliminary examination by Michael Beug during foray, later examination by Judy Roger & Joe Cohen on 21 May):

  • inamyloid;
  • Size: 13-17.5 × 5 µm (Judy Roger, Joe Cohen); roughly 9 × 4 µm; (Michael Beug)
  • Shape: smooth, no germ pore;
    cylindrical (Judy Roger, Joe Cohen); variously spindle, almond, or elliptical (Michael Beug)
  • Sterigmata exceptionally long
    ITS GenBank Number: KT968607
    RPB2 GenBank Number: KT968661

Species Lists

Images

521236
IMG_0199.jpg
Copyright © 2015 Joseph D. Cohen
521237
IMG_0200.jpg
Copyright © 2015 Joseph D. Cohen
522088
DSC_0040.jpg
Copyright © 2015 Judy Roger
522089
DSC_0041.jpg
Copyright © 2015 Judy Roger
522090
DSC_0042.jpg
Copyright © 2015 Judy Roger
522091
DSC_0043.jpg
Copyright © 2015 Judy Roger
with Congo Red
522092
DSC_0044.jpg
Copyright © 2015 Judy Roger
with Congo Red
522093
DSC_0047.jpg
Copyright © 2015 Judy Roger
gill edges
522157
IMG_0236.jpg
Copyright © 2015 Joseph D. Cohen
gill with KOH; red arrow shows area of color change

Proposed Names

OMS
-15% (3)
Recognized by sight
OMS
4% (4)
Recognized by sight: Large size, stocky, Tricholoma-/Hygrophorus-like shape
Used references: Gibson, Matchmaker: Mushrooms of the Pacific Northwest, Version 2.2 (Apr. 2014) ;(application, downloadable at http://www.svims.ca/council/matchmaker.htm); Bigelow, H.E., & Smith A.H., 1973. Cantharocybe, a new Genus of Agaricales. Mycologia. 65:485-488
Based on microscopic features: Spore shape and size: smooth, cylindrical; 13-17.5 × 5 µm
Based on chemical features: gills yellowish with KOH
-45% (2)
Recognized by sight: See Lab Report and comment below
28% (1)
Eyes3
Recognized by sight: serrated gills, tough stocky fb
Based on chemical features: closest DNA match to other sp. of Neolentinus.

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Neolentinus
By: Joe Cohen (Joseph D. Cohen)
2016-05-19 21:12:25 CEST (+0200)

Debbie:
Quite right. Don’t know what I was thinking.
Neolentinus probably the best current guess given the DNA (assuming that the close GenBank sequences are correctly ID’d).

color me confused.
By: Debbie Viess (amanitarita)
2016-05-19 17:51:20 CEST (+0200)

are we not calling this some sort of Neolentinus? That genus is NOT included in the family Hygrophoraceae.

Why not just stick with Neolentinus sp. for now? It certainly has those tell-tale serrated gills and tough, stocky form.

N. lepideus?
By: Joe Cohen (Joseph D. Cohen)
2016-05-18 05:37:44 CEST (+0200)

The sequence results below were discussed at the 2016 Spring Key Council Foray.
Nobody supported the original determination of Cantharocybe gruberi. But
everyone who spoke about this was adamant — based on photos of the specimen — that it was not Neolentinus lepideus. There were suggestions that it could be N. ponderosus, but more people felt we were dealing with a new taxon.

BLAST results lab report
By: Joe Cohen (Joseph D. Cohen)
2016-05-18 05:37:15 CEST (+0200)

Thanks to Kelli Van Norman and ISSSSP for funding the sampling:

BLAST results for OMS Mycoflora group samples
compiled by Matt Gordon, Molecular Solutions, LLC, 11/17/2015
y015 identified as Cantharocybe gruberi MO 204417 OMS 1000043
The top ITS matches are all Neolentinus lepideus, but the range of similarity is 92% to 97%. Similarity to Cantharocybe gruberi was only 70%. 97% isn’t a great match, but there seems to be a lot of ITS variation in the species. The top 2 rpb2 matches are also Neolentinus lepideus (99% and 92%). Does Neolentinus lepideus make sense for this collection taxonomically?

nor do we, Adolf.
By: Debbie Viess (amanitarita)
2015-05-27 21:45:27 CEST (+0200)

Joe is just trying to make sense of the “serrated” gills that were present in the most recent OR collection.

This is a very interesting mushroom that is not often collected. Therefore, we are still building a working species concept. “Eroded” gills seem to be one of the possible manifestations.

I looked hard at an enlarged image of the gill edges …
By: Debbie Viess (amanitarita)
2015-05-27 20:58:52 CEST (+0200)

they seem more eroded than serrated.

I also noticed those crazy long sterigmata in my micrograph of this species, collected in the Sierra several springs ago:

http://mushroomobserver.org/image/show_image/43965?obs=21104&q=2ZfB0

Gill edges on other specimens
By: Joe Cohen (Joseph D. Cohen)
2015-05-23 01:44:55 CEST (+0200)

Adolf:

Thanks for the comment. We also found this species last year about 5 miles from Suttle Lake. See Observation 166396

A few questions:

1. The Bigelow & Smith description of the holotype says: “Lamellae … edges even.” Does this mean the gill edges are not serrated?

2. Did Oluna create the drawing which goes with Observation 65653? And if yes, would she likely have noted serrated gills?

(I’m asking because the gill edges on this Observation 204417 are clearly serrated. See Image 522093.)

Regards,
- Joe

Created: 2015-05-17 00:52:13 CEST (+0200)
Last modified: 2016-05-19 21:10:21 CEST (+0200)
Viewed: 279 times, last viewed: 2016-10-24 04:25:39 CEST (+0200)
Show Log