Observation 204749: Amanita sect. Vaginatae sensu Zhu L. Yang
When: 2015-05-21
No herbarium specimen
0 Sequences

Notes:
Smaller gray Amanita sp. found near young oak. No ring. Sacklike volva. Margins striated.

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Eye3 = Observer’s choice
Eyes3 = Current consensus

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Let me try again with regard to what we have from the genes derived from the…
By: R. E. Tulloss (ret)
2017-03-21 10:54:11 CDT (-0500)

material of 204746 and 204749. I screwed up previously.

204746: We have a reasonably good nrLSU sequence. We have the end of the nrITS sequence (not enough to post in GenBank) attached to the initial 50 or so characters of nrLSU. This is how we can see the typical penetratrix-group 5’ terminal (“left hand end”) motif.

204749: We have what appears to be a damaged (or, otherwise, strongly differentiated) nrLSU. That doesn’t BLAST to the same species as the nrITS sequence does. The nrITS sequence is of good quality and has the aforementioned motif attached to its end.

So the two taxa are differentiated rather strongly. Future evidence (I hope) will show us whether or not we got the “clearest possible picture” from the material in hand or whether we can improve our understanding of what’s going on with these two probable species.

Sorry for the confusion. The useful outcome is that now both collections are listed on the Amanitaceae Studies’ penetratrix technical tab as lesser known members of the aforementioned “group.” The are listed by my accession numbers 685-3 (MO #604746) and 685-4 (MO #604749).

Very best,

Rod

Let me try again with regard to what we have from the genes derived from the…
By: R. E. Tulloss (ret)
2017-03-21 10:53:41 CDT (-0500)

material of 204746 and 204749. I screwed up previously.

204746: We have a reasonably good nrLSU sequence. We have the end of the nrITS sequence (not enough to post in GenBank) attached to the initial 50 or so characters of nrLSU. This is how we can see the typical penetratrix-group 5’ terminal (“left hand end”) motif.

204749: We have what appears to be a damaged (or, otherwise, strongly differentiated) nrLSU. That doesn’t BLAST to the same species as the nrITS sequence does. The nrITS sequence is of good quality and has the aforementioned motif attached to its end.

So the two taxa are differentiated rather strongly. Future evidence (I hope) will show us whether or not we got the “clearest possible picture” from the material in hand or whether we can improve our understanding of what’s going on with these two probable species.

Sorry for the confusion. The useful outcome is that now both collections are listed on the Amanitaceae Studies’ penetratrix technical tab as lesser known members of the aforementioned “group.” The are listed by my accession number 685-3 (MO #604746) and 685-4 (MO #604749).

Very best,

Rod

Nope. I gave you a bad lead. <<<<——-EDITED
By: R. E. Tulloss (ret)
2017-03-21 10:16:53 CDT (-0500)

The sequences we got from the two taxa with regard to the nrLSU locus are quite dissimilar.

Very best,

Rod

I don’t
By: Logan Wiedenfeld (LoganW)
2017-03-21 10:12:18 CDT (-0500)

think so, Rod. obs 204747 just seems like a more impressive and robust taxon. I could be wrong, since this one is immature; but if I had to guess, I’d say the two are distinct.

Logan, do you think that MO 204747 is the same species as you have in…
By: R. E. Tulloss (ret)
2017-03-21 10:02:25 CDT (-0500)

this observation?

Very best,

Rod

A report. <<——-EDITED
By: R. E. Tulloss (ret)
2017-02-13 21:48:49 CST (-0600)

We have an nrITS sequence. The nrLSU “left hand end” motif is TCT… as in the provisional series Penetratrices. Unfortunately, the nrLSU as a whole may have been damaged and is not comparable to other nrLSUs of the penetratrix group.

The best thing going is that we seem to have found still another one of the Penetratrices group.

Very best,

Rod

Thanks Logan,
By: groundhog
2015-06-05 11:51:59 CDT (-0500)

This material has been received and accessioned to Rod’s herbarium.
Thanks,
Naomi

This is another collection that I would like to see…
By: R. E. Tulloss (ret)
2015-05-21 12:14:08 CDT (-0500)

if that is possible.

Very best,

Rod

Created: 2015-05-21 11:49:17 CDT (-0500)
Last modified: 2017-03-21 10:25:50 CDT (-0500)
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