Observation 204771: Ramaria Fr. ex Bonord.
When: 2015-05-15

Notes: Habitat: between a dead standing conifer and a grand fir, several grand fir in immediate area with Doug-fir and Ponderosa Pine nearby. Stipe flesh very slowly and weakly amyloid. Basidia clamped, spores averaged 9.3 × 3.1, no spore ornamentation noted. Taste and odor not distinctive. Light red bruising noted in one of the photos but didn’t notice when collected.

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Eye3 Eyes3
Recognized by sight
27% (1)
Based on chemical features: See Lab Report in Comments

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Eye3 = Observer’s choice
Eyes3 = Current consensus

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GenBank numbers
By: Joe Cohen (Joe Cohen)
2016-05-17 23:59:27 EDT (-0400)

GenBank numbers for the sequences mentioned in the Lab Report. (See Comment below.)
ITS GenBank Number KT968608
RPB2 GenBank Number KT968662

BLAST results lab report
By: Joe Cohen (Joe Cohen)
2016-05-17 23:56:02 EDT (-0400)

Thanks to Kelli Van Norman and ISSSSP for funding the sampling:

BLAST results for OMS Mycoflora group samples
compiled by Matt Gordon, Molecular Solutions, LLC, 11/17/2015
y016 identified as Ramaria rasilispora MO 204771
There is no R. rasilispora sequence in GenBank, so these will be the first. BLAST showed one 98% match with a specimen identified as Ramaria thiersii. Not sure about the identification of that one. R. thiersii and R. rasilispora are both in subgenus Leaticolora, so this makes some sense. The odd thing is that there are no other Ramarias more than 90% similar in ITS, so this (and the thiersii in GenBank) is something of an outlier. I found what I thought was a R. rasilispora this June near Carl Lake, and the sequence of that one was 99% similar to y016, so that is the closest match (haven’t put it in GenBank yet).

Created: 2015-05-21 23:22:02 EDT (-0400)
Last modified: 2016-05-18 00:01:16 EDT (-0400)
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