Observation 205108: Cordycipitaceae sensu lato
When: 2015-05-25
(45.2454° -121.5776° 1493m)
Herbarium specimen reported

Notes:
MO-205108
Collector(s): Joseph D. Cohen
Determined: 2015-May-27
Determiner: Joseph D. Cohen
Original Determination: Cordycipitaceae Sensu Lato
Habitat: conifer forest of Tsuga mertensiana (Mountain Hemlock) and Abies amabilis (Pacific Silver Fir); bushes dominated by Huckleberry
Aspect: none
Growth Habit: Single
Substrate: Duff
Closest plant: Abies amabilis (Pacific Silver Fir)
Other Habitat & Locality Notes: Found within 10m of FR 4891 (Bonney Meadows Rd.). Geolocation and elevation estimated from Barlow RD map, +/- 800m; elevation +/- 60m.
Odor:
Taste:
Spore print:
Other Specimen Notes: Cap was orange (lighter than shown in photo) when collected.
Looks just like Ophiocordyceps gracilis, which was the original determination based on macro features (cap color, larval substrate), but size of specimen spore segments is too long for that taxon

Proposed Names

14% (2)
Used references: Gibson, Matchmaker: Mushrooms of the Pacific Northwest, Version 2.2 (Apr. 2014) (application, downloadable at http://www.svims.ca/council/matchmaker.htm)
46% (2)
Eye3
Recognized by sight: Combination of substrate and spore size does not match Ophiocordyceps gracilis or any or anything else found in PNW (per Matchmaker)
Used references: Gibson, Matchmaker: Mushrooms of the Pacific Northwest, Version 2.2 (Apr. 2014) (application, downloadable at http://www.svims.ca/council/matchmaker.htm)
Based on microscopic features: See notes
56% (1)
Recognized by sight
57% (1)
Eye3 Eyes3
Recognized by sight: Bright orange cap, substrate is insect larva
Used references: Gibson, Matchmaker: Mushrooms of the Pacific Northwest, Version 2.2 (Apr. 2014)
Based on microscopic features: Spores cylindrical in chains of 8 to >20 per chain; each segment in chain about 17.5 µm long

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Failed Sequence
By: Joe Cohen (Joseph D. Cohen)
2016-05-17 23:01:27 EDT (-0400)

Thanks to Kelli Van Norman and ISSSSP for funding the sampling:

BLAST results for OMS Mycoflora group samples
compiled by Matt Gordon, Molecular Solutions, LLC, 11/17/2015
y011, identified as Cordycepitaceae MO 205108
I tried 2 separate extractions from this specimen but couldn’t get a clean sequence. There was always at least one other sequence present, indicating that another ascomycete has colonized the sporocarp (I used ascomycete-specific primers rather than the general fungal primers). One way to get the Cordyceps sequence would be to use genus or family-specific primers for Cordyceps, but I could not find any that had been published.

Created: 2015-05-27 09:14:41 EDT (-0400)
Last modified: 2015-06-07 18:19:16 EDT (-0400)
Viewed: 99 times, last viewed: 2016-06-30 04:27:23 EDT (-0400)
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