Observation 208825: Amanita justicei Tulloss & Kudzma nom. prov.
When: 2015-07-04
38.528° -90.527°
0 Sequences

Proposed Names

39% (2)
Eye3
Recognized by sight: Photo of the volva did not turn out. Thin, sheathing sack.
ret
54% (1)
Eyes3
Recognized by sight: see comment
Based on chemical features: see comment
ret
54% (1)
Based on chemical features

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Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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We are working on a quick and dirty tree to get a hint of what possible realtionships
By: R. E. Tulloss (ret)
2017-04-21 23:33:29 IST (+0530)

might be extractable from nrLSU data. We discovered that we can now get a better view of the placement of the present collection. We have to consider that it is also genetically similar to Amanita penetrans.

This becomes an unresolved issue. Maybe we can blame this all on the big hole in the “middle” of the nrLSU sequence we got from the present material. It would have helped if we could have gotten nrITS; however, we got no useful data there. Maybe we’ll have time to try another gene, but we’ll need to get a great number of sequences for such a gene ourself because. Amanita sect. Vaginatae has not been studied very much from a genetic point of view. We can expand our morphological studies of this material.

Very best,

The sequences for this collection were largely fragmentary.
By: R. E. Tulloss (ret)
2017-02-07 07:39:18 IST (+0530)

A segment of 385 characters near the start (5’ end) of nrLSU was an exact match for justicei sequences in the same region.

There is no question that the species is assignable to series Penetratrices.

After a significant gap, in nrLSU, we derived a 771 character sequence. This was a nearly exact match for the same 771 character region in the nrLSU of justicei.

Imperfections in the match: There is a one character gap that could be due a PCR error (one less “T” in a multi-“T” repeat). It could also be natural variation. Two characters were ambiguous and a “partial match” with other justicei sequences. Two characters did not match; these were located near the 3’ end of apparently useful data from a LR3R read.

The photograph is consistent with __justicei__’s cap browning with age.

So I’ll propose that the present species is A. justicei although there is further work necessary.

Very best,

Rod

There are a couple of things… >>EDITED QUITE A BIT
By: R. E. Tulloss (ret)
2016-12-25 22:45:03 IST (+0530)

…that I am beginning to notice about MO vouchers.

On the 21st of this month, I got ca. 250 “reads” from sequencing that is based on the molecular work of Linas Kudzma. He warned me in advance.

I am beginning to realize that I can relatively quickly scan for sequences and photos on MO that combine to reveal a species “concept” that I have already hypothesized.

Then there are the sequences and photos that are just a tad off from what I think I knew about a probable species, then there are sequences for gene A that very closely match a sequence I have seen before and, also, is paired with (come from the same sample) a sequence for gene B that is not close to gene B sequences from the same taxon “concept.” Merge with this the growing data that suggest I can’t trust data from gene B to serve all the “ID purposes”.

Hence, these genes cannot serve (even as a pair) to identify a new species by simple comparison to other genes.

Dr. Kudzma is in the process of expanding his work to the derivation of sequences from other genes (especially those that encode proteins rather the blueprint for parts of a ribosome).

It will be an interesting 2017. I am glad you have the patience to deal with all the things that affect our processing of the material you have sent me. I do the best I can.

When he worked at the herbarium in Leiden, Dr. Bas had a sign on his office door. I think of it frequently. In translation, it said approximately: “I do what I can. What is not done I could not do.”

Very best,

Rod

Thank you too!
By: Patrick Harvey (pg_harvey)
2016-12-25 21:23:17 IST (+0530)

I really appreciate occasional notes about your findings. I’ll be sure to get you more of this section in the coming year.

Happy holidays to you both!

Patrick Harvey

Further work remains to be done; however, we have received sequences for…
By: R. E. Tulloss (ret)
2016-12-25 09:42:29 IST (+0530)

this material and other MO vouchers in recent days. This falls in a group that seems rather close to “sp-T06”. We have to work on comparisons of this group before we can know a little of what is going on. More data to come.

Thanks for sending this material, Patrick.

Very best,

Rod

Thanks!
By: groundhog
2015-07-28 21:12:19 IST (+0530)

We have received the material and accessioned it to Rod’s Herbarium. We have scheduled it to be sequenced.
Thanks,
N

Thank you.
By: R. E. Tulloss (ret)
2015-07-05 09:33:38 IST (+0530)

Rod

That’s a good question!
By: Patrick Harvey (pg_harvey)
2015-07-05 00:49:56 IST (+0530)

I am not really sure. The soil I pulled it out of is also kind of grayish when dried. That end of the (now very thin) stalk does look gray. Sorry I didn’t get a good shot of the volva fresh on this one, but it was white then as I recall.

Is the volval sac becoming grayer with time?
By: R. E. Tulloss (ret)
2015-07-05 00:23:26 IST (+0530)

Rod

Created: 2015-07-04 23:29:31 IST (+0530)
Last modified: 2017-04-21 23:34:14 IST (+0530)
Viewed: 103 times, last viewed: 2017-09-15 10:22:11 IST (+0530)
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