Observation 208874: Lanmaoa G. Wu & Zhu L. Yang
When: 2015-07-04
(39.9472° -74.9786° 40m)

Notes: > Two basidiomata growing in grass under mature pin oaks.
> One fb had a gray pore surface probably caused by hypomyzation.
> KOH = rusty orange on cap and pinkish-orange on context; NH4OH = a greenish-blue flash on contact with cap with a purple halo around the liquid, resolving to rusty orange; negative to pale yellow on context; FeSO4 = grayish-olive on cap and grayish on context. The macrochemical tests on context were in line with those for B. bicolor.

Molecular Data Discussion:
>A clean and contiguous 1446 bp nrLSU sequence was obtained for this material
>A GenBank BLAST search of the full-length sequence did not produce anything meaningful. As a matter of fact, not a single taxon from this section of the Boletacea was recovered from the first 100 alignments!
>A GenBank BLAST search of a shorter fragment of my sequence (the first 981 bps) yielded a 100% match (score = 1626; 880 bp overlay, 89% coverage) with the Lanmaoa pseudosensibilis voucher DS615-07, accession #KF030257. The matching sequence was associated with the following publication:
“Phylogenetic overview of the Boletineae”: Nuhn, M.E., Binder, M., Taylor, A.F., Halling, R.E. and Hibbett, D.S.; Fungal Biol 2013, 117 (7-8), 479-511.

Conclusion:
While I don’t think this bolete is L. pseudosensibilis or B. pallidoroseus, both the morphology and the molecular evidence point toward this being a closely related taxon, possibly belonging in Lanmaoa. It appears that the nrLSU gene alone is probably not capable of phylogenetically resolving boletes in this section.

Images

534996
Note the parasitized pore surface of the sporocarp on left
534997
After a while the blue color resolved to cherry-brown
534998
Discoloration as seen ~30 sec after dissection
534999
Discoloration as seen ~2 min after dissection
535000
Discoloration as seen ~6 min after dissection
535001
The original cap color was probably pale rose-red or reddish-tan

Proposed Names

28% (1)
Recognized by sight
-1% (2)
Recognized by sight: Color scheme and bluing pattern match mature pallidoroseus.
Used references: BRB
Based on chemical features: KOH reactions match fairly well, NH4OH reactions do not match.
61% (2)
Eye3 Eyes3
Based on chemical features: LSU sequence close to L. pseudosensibilis

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

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Hi, Dave
By: I. G. Safonov (IGSafonov)
2016-01-18 07:43:50 PST (-0800)

I don’t recall seeing your observation before, so thanks for pointing it out to me. Yes, they do look very similar, and both have too much red on the stipe to be L. pseudosensibilis. The conspicuous reddening/browning of the exposed flesh where it stained blue before is not a fact mentioned by any of the sources I checked, but I recall G. Lincoff talking about it at COMA last year. Perhaps it’s one of the unifying “morphological themes”, along with others in a consistent set of observable macro characters, tying L. pseudosensibilis and other closely related taxa that pose as such together.

Hi, John
By: I. G. Safonov (IGSafonov)
2016-01-18 06:54:20 PST (-0800)

Thanks for your comment. I am yet to see gregarious growth like you mentioned for any bolete, but in in the past one of the parks in Hunterdon county in NJ produced tons of bicolor-like taxa in its oak groves (the last great bolete flush was in August 2011, but since that the place has been “dead”). I used to eat them with impunity, too, thinking it’s all B. bicolor, but then I figured out that some were the true bicolor and others were B. pallidoroseus (the odor was unmistakable) and possibly something else. The same place had also yielded L. pseudosensibilis, which I believe I posted on MO. I also procured an nrLSU sequence for obs215637, which fits my concept of L. pseudosensibilis, and will be posting a discussion comparing the two later on.

Looking through my old observations…
By: Dave W (Dave W)
2016-01-17 19:36:16 PST (-0800)

this looks like probably the same as obs 74031 . The notes mention the strong bluing on the pores that fades to brown. This observation (74031) was made in Moon Lake Park, an area with mainly oak-dominated woods and wood borders near open areas.

I have seen this
By: John Plischke (John Plischke)
2016-01-17 18:35:42 PST (-0800)

I have seen this one at Saint Clair Cemetery. Growing under pin oak by the 100’s. It has not appeared in the last several years. 20 years ago I used to think it was bicolor and ate it and it was one of the best tasting boletes that I ever tried. I few years later I realized it was not bicolor but could not figure out what they were and I stopped eating them. I always thought the caps becomes somewhat brown with age more so that bicolor or pseudosensibilis

DNA discussion posted
By: I. G. Safonov (IGSafonov)
2016-01-17 00:01:39 PST (-0800)
Dave,
By: I. G. Safonov (IGSafonov)
2015-07-04 20:41:17 PDT (-0700)

I did consider B. pallidoroseus, but there was no beef bouillon odor typically associated with this species.

Created: 2015-07-04 19:31:17 PDT (-0700)
Last modified: 2016-08-09 15:48:45 PDT (-0700)
Viewed: 96 times, last viewed: 2016-11-15 12:53:13 PST (-0800)
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