Observation 209351: Amanita “sp-MO04”
When: 2015-07-09

Notes: Growing all by itself in mixed hardwoods.
Standing very tall on pretty white stipe.
This is 1 of 2 found on todays hike about 1 mile apart that I think are the same species. A separate observation will be made of the second one.

Proposed Names

81% (1)
Based on chemical features: This collection is the basis for the concept of the temporary code name.

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Eye3 = Observer’s choice
Eyes3 = Current consensus


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very nice
By: John McDonough
2016-06-04 09:49:29 CST (+0800)

Looking good. Glad to see you are making some progress!

The web page is looking better with two images and more text.
By: R. E. Tulloss (ret)
2016-05-31 07:30:14 CST (+0800)
Thanks, John.
By: R. E. Tulloss (ret)
2016-04-04 05:35:26 CST (+0800)

That’s what I thought, but better not to make assumptions.

Very best,


Yes sir,
By: John McDonough
2016-04-04 05:23:17 CST (+0800)

they were collected on the same day during the same hike about 1 mile apart from the same forested region.

Can I use the same forest description for this species as I did for A. penetrans?
By: R. E. Tulloss (ret)
2016-04-04 03:48:46 CST (+0800)

We got a very messy nrITS for this species. But we got a very nice nrLSU sequence. I’m going to submit the latter to GenBank.

It is quite possible that this species is one of those amanitas that have more than one nrITS sequence, which produces a largely garbled result.

Very best,


well, now i feel bad
By: John McDonough
2015-10-24 07:28:02 CST (+0800)

that I did not collect some others I saw that day…….I will get you some more from that area next season.
Have a great weekend!

Based on one sample (note!) this is distinct from A.penetratrix.”
By: R. E. Tulloss (ret)
2015-10-21 03:06:49 CST (+0800)

The cap color is certainly quite different from that of penetratrix, with three irregular color zones and yellow pigment that is utterly missing in the material of “penetratrix” from Connecticut. We have a pretty good nrITS sequence for the material of this observation, and it differs from that of the white to gray-brown CT material.

There is also something very unusual (“in my experience,” I hasten to add):

In all the amanita “proposed fungal barcode” (nrITS) sequences I’ve seen, the 14 characters immediately following the nrITS sequence are “TTGACCTCAAATCA.” Thia character string at the beginning (5’ end) of nrLSU (long subunit gene)—a very conserved part of that gene. In penetratrix, the corresponding string has an inserted “C”: TCTGACCTCAAATCA. An insertion of a character in the second position is something common in sequences of Limacella, but not in Amanita (again, “so far as I know”).

NOTE: I am not saying that this species is a Limacella or even closely related to Limacella. It obviously is a species of section Vaginatae. I just want to say that the little oddity of penetratrix is NOT present in the present species; however, it is present in the other brown penetratrix-like material of MO #209352, which, however, (again based on a single collection) also appears to be genetically distinct from penetratrix genetically based on the nrITS.

So there are at least three penetratrix-like taxa…apparently with different cap colors.

Very best,


DNA data has been received.
By: R. E. Tulloss (ret)
2015-10-21 02:08:40 CST (+0800)

More in a little bit. This might be distinct from the east coast penetratrix. The cap color sure is different. Looks there will be a genetic difference, too.

Very best,


Thanks, John
By: groundhog
2015-07-21 23:38:08 CST (+0800)

We have received this material and it has be accessioned into Rod’s herbarium.

Thanks John,
By: groundhog
2015-07-18 03:54:27 CST (+0800)

This material has arrived and had been accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing. Thanks for filling out the collecting notes and providing this material!
-Naomi (working with RET)

Appendix A2
By: John McDonough
2015-07-10 03:03:21 CST (+0800)

collecting notes filled out and material is in the dryer

By: John McDonough
2015-07-10 01:27:17 CST (+0800)

umbo is very firm and does require more pressure than I anticipated to make a dent with my thumb nail. the second specimen was more developed and I would call it mammilate or papillate or at least that is how I see it developing.
gills are white and not pink
“artifact” was debris and has been removed……its a jungle out there:)

Did a distinctive umbo develop above the stem?
By: R. E. Tulloss (ret)
2015-07-10 01:14:21 CST (+0800)

If so, is the umbo particularly dense and hard to cut through?

Very best,


This looks like something very interesting.
By: R. E. Tulloss (ret)
2015-07-10 01:13:14 CST (+0800)

The portion of the stem that was underground is unusual. The gills seem to have a pink tint (is that an artifact?).

I’d very much like to examine this material.

Very best,


Created: 2015-07-10 00:47:31 CST (+0800)
Last modified: 2016-04-04 03:55:26 CST (+0800)
Viewed: 153 times, last viewed: 2017-06-20 21:58:16 CST (+0800)
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