Observation 209863: Amanita “cattaraugana” Tulloss & Kudzma nom. prov.
When: 2015-07-12
(42.055° -78.6628° 550m)

Notes: These huge specimens were buried pretty deeply in the dirt. There were Oaks and Beech around as well as Maple and other hardwoods. I am fairly certain these were all the same species.

Proposed Names

ret
-27% (1)
Recognized by sight
ret
81% (1)
Eyes3
Based on chemical features: We received DNA from this material, Garrett, and it was “none of the above.” It’s an undescribed species that we know only from one other collection … by Linas Kudzma. With your permission, I’ll creat a new taxon page with images from this observation.

Please login to propose your own names and vote on existing names.

Eye3 = Observer’s choice
Eyes3 = Current consensus

Comments

Add Comment
Nice work, Garrett…
By: Dave W (Dave W)
2016-05-20 22:42:45 EEST (+0300)

collecting/preserving several specimens of this. Excavating the deeply-buried types requires a bit of dedication to the task at hand.

GenBank accession numbers are posted,…
By: R. E. Tulloss (ret)
2016-05-20 21:20:35 EEST (+0300)

but there will be no links to GenBank until the sequences are posted publicly by GenBank editors.

Thanks again; this is a cool species.

Very best,

Rod

Thanks, Garrett.
By: R. E. Tulloss (ret)
2016-05-06 16:26:06 EEST (+0300)

I picked the first shot.

Very best,

Rod

Reply to the question about images for your website,
By: Garrett Taylor (cappy)
2016-05-06 06:14:56 EEST (+0300)

go for it!

I am depositing the sequences in GenBank and decided to go with…
By: R. E. Tulloss (ret)
2016-05-05 18:30:39 EEST (+0300)

a provisional name: Amanita cattaraugana.

It honors Cattaraugus Creek, Cattaraugus County, and the Cattaraugus Reservation (Seneca Nation).

Very best,

Rod

This is great!
By: Garrett Taylor (cappy)
2016-04-29 05:11:40 EEST (+0300)
All the specimens had the same “proposed fungal barcode” sequence.
By: R. E. Tulloss (ret)
2016-04-28 17:11:55 EEST (+0300)

And they all agreed with a second collection from NW New Jersey (Hunterdon Co.). Moreover, the the nrLSU sequences for both collection match very well.

We will be submitting the sequences to GenBank shortly.

Very best,

Rod and Naomi

Update
By: groundhog
2016-01-04 19:14:22 EET (+0200)

Hi Garrett,

We decided to sample all cap pieces in this collection in case it happened to be composed of multiple species. It turns out that all pieces of this collection are consistent with representing a single ‘proposed fungal barcode’. As Rod commented, the nrITS and nrLSU sequence from this collection match well to a collection made by Linas Kudzma from Hunterdon Co., NJ. In addition the odd beginning of the LSU sequence that is seen in sequences of A. penetratrix (and a few other sp.) was not observed here.

Thanks for the material!
Naomi

In the dried material there are two 1/4 caps with a very distinctive umbo…
By: R. E. Tulloss (ret)
2015-11-27 05:55:14 EET (+0200)

and two 1/4 caps that have a flatter cap that seems more fully expanded. I think it would be reasonable to resample one of the peaked cap parts and one of the flatter cap parts to see if we get two different sequences.

I think I’ll plan on taking that route.

A very interesting collection.

Thanks again.

Rod

You are talking about matching the mushroom to one of the images
By: Garrett Taylor (cappy)
2015-11-27 05:38:31 EET (+0200)

associated with this observation 209863 and not some other observation, correct? In my mind, the only image which could possibly be a different specimen would be the cover picture with the nibbled stipe, of course it’s the nicest picture. The others were on the other side of the access path in closer proximity and were identical macroscopically. The nibbled one had a slight orange-y tint to the brown on the stipe, was not as deeply buried, though it was growing in soil with more clay content up slope thirty or forty yards from the nearest of the others. The others were along the path (slight inclined) maybe twenty yards separating all of half dozen(?) of them, in more loose, possibly sandy soil perhaps due to the adjacent path. I didn’t nibble it…then anyway, I promise!

Correction.
By: R. E. Tulloss (ret)
2015-11-26 22:04:27 EET (+0200)

The previously known collection with the same sequence, is from Hunterdon Co., NJ.

R

I think I better restrict the meaning of “sp-N64” to the specimen that we …
By: R. E. Tulloss (ret)
2015-11-26 18:29:47 EET (+0200)

sequenced. Since you mention it in your original notes, maybe we should take a conservative view that different specimens may be different taxa. The other collection with the same DNA had a thicker stipe that was not so deeply buried in the ground. Could all be within a “range of variation” or not. The sequence from your material that we have at present is the “proposed fungal barcode” gene.

I’m going to look at the material you sent to see if I can associate it with one of your images….

Very best,

Rod

Thanks Garrett,
By: groundhog
2015-07-21 18:24:34 EEST (+0300)

This material has been received and accessioned to Rod’s herbarium. We have scheduled it for DNA sequencing.
-Naomi

Also, Yves emphasize the yellow pigment on the cap and stipe decorations in…
By: R. E. Tulloss (ret)
2015-07-18 00:59:25 EEST (+0300)

A, elongatior. Again, I think that this characteristic is lacking in the material photographed for this observation.

Hence, I am going to proposed “penetratrix” as a possible determination.

We have scheduled your material to be sampled for DNA sequencing.

Thanks again.

Very best,

Rod

We were processing your dried material this afternoon.
By: R. E. Tulloss (ret)
2015-07-18 00:56:14 EEST (+0300)

We very much appreciate your sending it. I noticed that Yves’ description of A. magna emphasizes a very dark umob and a dark ring-like zone positioned over the inner ends of the marginal striations. I don’t think that this material satisfies that part of the original description of A. magna.

Rod

That’s typical of “penetratrix.”
By: R. E. Tulloss (ret)
2015-07-13 18:51:16 EEST (+0300)

R

My crumby luck with this species goes on and on and on and….
By: R. E. Tulloss (ret)
2015-07-13 17:49:17 EEST (+0300)

I’ll really be interested to see what survived.

Very best,

Rod

Yes the cap material was very tough through the umbo.
By: Garrett Taylor (cappy)
2015-07-13 16:19:52 EEST (+0300)

Only one made it to the dehydrator one from the cover I believe. I had them in the car while I was at work so a couple of the stalks started getting slimy.

I want to be clear that I am not contesting the name “magna” for this material..
By: R. E. Tulloss (ret)
2015-07-13 13:26:07 EEST (+0300)

Perhaps my provisional name applies to the same taxon as Yves’. At present, I don’t know.

Very best,

Rod

I think this is what I have been calling Amanitapenetratrix.”
By: R. E. Tulloss (ret)
2015-07-13 13:19:59 EEST (+0300)

Is the umbo very, very firm? (Significantly harder to cut through than any other part of the fruiting body.)

I would very much like to see all or part of your collection of this material. I have previously posted on MO about my bad luck drying “penetratrix.” So I have been on the look out for more material.

Very best,

Rod Tulloss

Created: 2015-07-13 06:22:02 EEST (+0300)
Last modified: 2016-05-05 18:27:13 EEST (+0300)
Viewed: 243 times, last viewed: 2016-11-09 12:57:22 EET (+0200)
Show Log