Observation 210760: Boletus pallidoroseus Both

Notes:
This was found in exactly the same spot as obs 103977. The gestalt morphology and the odor match those of pallidoroseus for both observations.

Molecular Sequencing Results & Discussion (most recent edit/update 11-Feb-18):
> A clean and contiguous nrLSU sequence of 1446 bps was obtained from this material. There are two haplotypes present differing only in the overall length – the second haplotype has an extra “G” at position 527. Other than that, the two sequences are perfectly identical. There are three (3) ambiguous characters present – one “R” (A/G) and two “Y” (C/T). A GenBank BLAST search of the first 981 characters of Haplotype 1 produced the following top-scoring hits:
1) Boletus bicolorTH6933 (#AY612800) = 926/930 = 99.6% similar (all 4 mismatching bases are ambiguous). This voucher has clearly been misidentified.
2) Boletus pallidoroseus snHor01 (#KF030305) = 876/880 = 99.5% similar (3/4 mismatching bases are ambiguous). This is a trusted voucher.1
3) Lanmaoa carminipes MB06-001 (#JQ327001) = 876/881 = 99.4% similar. This is a trusted voucher.1,2,3
Ten out of twelve next hits are trusted accessions of Lanmaoa species that are 98.6—>96.8% similar.
> A clean and contiguous TEF-1-α sequence of 610 bps was also obtained from this collection. There is a single ambiguous character, a “Y”. A GenBank BLAST search of this sequence revealed that the first fourteen (14) hits are all accessions of Lanmaoa species from North America and Asia, with similarity in the 99.8—>95.3% range. Surprisingly, the top hit at 99.8% is Lanmaoa pseudosensibilis DS615-07 (#KF030407), a trusted voucher. 1,2,3
It is interesting that alignment of the nrLSU sequence of DS615-07 (#KF030257) with that of this observation (210760) shows them to be only 96.8% similar. At the same time KF030257 is a perfect match to my own vouchers of pseudosensibilis, obs 208874 and obs 215637. Since the LSU sequences of L. pseudosensibilis and B. pallidoroseus are very different, the same should be expected of their TEF-1 reads. Therefore, unless the two species naturally have identical TEF-1 sequences, I suspect that KF030407 should not be associated with L. pseudosensibilis DS615-07.
Apart from the single ambiguous base, this sequence is a perfect match to that of my other B. pallidoroseus voucher, obs 283635. Lastly, comparison with the TEF-1 of obs 247881 shows that other than the indicated ambiguities in the two reads (5 in 247881) they are identical, too.
> The above results strongly suggest that Boletus pallidoroseus is a bona fide member of Lanmaoa. Ergo, I propose Lanmaoa pallidorosea (Both) Safonov comb. prov.
> Unfortunately, the type of B. pallidoroseus collected by Ernst Both has not been sequenced yet. A thorough molecular characterization of the type collection is obviously highly desirable.

1“Phylogenetic overview of the Boletineae”, Fungal Biol. 2013, 117 (7-8), pp. 479-511.
2 “Molecular Phylogenetic Analyses Redefine Seven Major Clades and Reveal 22 New Generic Clades in the Fungal Family Boletaceae”, Fungal Diversity 2014, 69(1), pp. 93-115.
3 “One Hundred Noteworthy Boletes From China”, Fungal Diversity 2016, 81, pp.25-188.

Proposed Names

85% (1)
Eye3 Eyes3
Recognized by sight: Gross morphology and a strong beef bouillon odor; have previously collected this taxon at the location
57% (1)
Based on chemical features: Analysis of nrLSU & TEF-1-alpha sequences in GenBank

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Eye3 = Observer’s choice
Eyes3 = Current consensus

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DNA sequencing results and discussion UPDATED
By: I. G. Safonov (IGSafonov)
2017-02-04 01:08:00 CST (-0600)

Created: 2015-07-20 11:21:36 CDT (-0500)
Last modified: 2018-02-11 16:03:06 CST (-0600)
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